局部注射鼠神经生长因子对家兔眼外肌的形态学影响  

Changes of extraocular muscle after single direct injection of nerve growth factor in rabbits

作　　者：王飞[1] 陈金卯[1] 赖丽萍[1] 

机构地区：[1]广西医科大学第一附属医院眼科,南宁530021

出　　处：《山东大学耳鼻喉眼学报》2012年第2期76-79,共4页Journal of Otolaryngology and Ophthalmology of Shandong University

摘　　要：目的探讨兔眼外肌局部注射鼠神经生长因子(NGF)后的组织学变化,探讨药物治疗斜视的新方法。方法以10只成年新西兰大白兔为实验对象,左眼上直肌注射0.05 mL(5μg)NGF(实验组),右眼上直肌注射0.05 mL生理盐水(对照组)。1周后获取上直肌,测量其湿重及肌肉横截面积,并行组织病理学检查。结果实验组肌肉横截面积较对照组明显增大,两组肌肉湿重未见明显不同。注射部位无明显炎性细胞浸润,未见眼外肌肌纤维变性、坏死等毒性反应。注射部位附近的巩膜和相应的色素膜也未见明显炎性变化。Van Gieson氏苦味酸酸性复红染色未见胶原异常增生。实验组较对照组纤维周径、大细胞和中细胞的比例差异有统计学意义,实验组眼外肌增生细胞核抗原阳性细胞的比例较对照组显著提高。结论眼外肌局部注射鼠神经生长因子对眼部组织无明显不良作用。鼠神经生长因子能促使眼外肌肌纤维快速增生,并能促进组织细胞分化。Objective To evaluate the morphometry changes of adult rabbit extraocular muscle after direct injection of nerve growth factor（NGF）.Methods In a self-control study,the left eyes of 10 adult rabbits were selected as the experimental group,and the right eyes as the control group.5 μg（0.05 mL） NGF was injected into the superior rectus muscle of each experimental eye,and the identical volume of sterilized saline into each control eyes.One week after injection,muscle wet weight and muscle fiber cross-sectional areas were examined.The morphology of the muscles both in the experimental group and the control group was studied by hematoxylin-eosin staining and Van Gieson staining under a light microscope,and the proliferating cell nuclear antigen（PCNA） positive cells in the superior rectus muscle were determined by immunohistochemistry.Results There was no significant difference in wet weight between the experimental group[（0.14±0.02）g] and the control group[（0.12±0.02）g].The myofibers mean cross-sectional area of the superior rectus muscle was（9.67±1.82） mm2 in the experimental group,and（6.97±1.90） mm2 in the control group,with a significant difference between them（t=2.79,P=0.02）.No obvious infiltration of inflammatory cells was seen in the muscles after injection.The perimeter of the superior rectus muscle was（167.20±35.55） μm in the experimental group and（127.44±33.64） μm in the control group,with a significant difference between them（t=3.06,P0.001）.The frequencies of large and middle fibers in the muscles were higher in the experimental group than in the control group（χ2=64.16,P0.001）.The proportion of PCNA positive cells was higher in the experimental group than in the control group（χ2=54.61,P0.001）.Conclusion Single direct injection of NGF into extraocular muscles is an effective and safe procedure.NGF can increase myofibers cross-sectional area of the extraocular muscle and promote the proliferation of extraocular muscle fibers.

关 键 词：眼外肌 神经生长因子 增生细胞核抗原 兔 斜视 

分 类 号：R777.4[医药卫生—眼科]