地衣芽孢杆菌P-104发酵生产γ-聚谷氨酸条件优化  

Optimization of γ-Polyglutamic Acid Production by Bacillus licheniformis P-104

作　　者：张业伟[1] 魏雪团[1,2] 胡中波[1] 罗明芳[3] 徐林[1,2] 刘会洲[2] 

机构地区：[1]中国科学院研究生院,北京100049 [2]中国科学院过程工程研究所,北京100190 [3]中国科学院微生物研究所,北京100101

出　　处：《过程工程学报》2012年第2期288-292,共5页The Chinese Journal of Process Engineering

基　　金：国家自然科学基金资助项目(编号:20967186)

摘　　要：对地衣芽孢杆菌P-104发酵合成γ-PGA的条件(接种时间、接种量和培养基组成等)进行了优化,并在发酵罐中进行了批式发酵实验.结果表明,该菌可利用合成培养基生产较高浓度超高分子量(大于2500kDa)的γ-PGA,最佳培养基组分为(g/L):葡萄糖80,谷氨酸钠70,柠檬酸钠10,(NH4)2SO410,MnSO40.15,MgSO40.8,K2HPO40.6,NaNO34.接种时间与量分别为8h和3%(ψ)、初始pH7.5条件下,37℃下180r/min摇瓶培养24h,发酵液中γ-PGA浓度可达44.7g/L,比生产速率为1.49g/(L·h),是已报道的同类比生产速率的2倍.采用优化培养基在6.6L发酵罐中批式发酵培养33h,γ-PGA浓度为32g/L,比生产速率为0.97g/(L·h).The fermentation conditions for γ-polyglutamic acid （γ-PGA） production with Bacillus licheniformis P-104 were optimized in flasks, and the mesoscale batch fermentation was performed in a 6.6 L fermentor. The results showed that high yield of g-PGA with ultra high molecular weight （over 2500 kDa） was obtained under the optimal conditions （g/L）： glucose 80, sodium glutamate 70, sodium citrate 10, （NH4）2SO4 10, MnSO4 0.15, MgSO4 0.8, K2HPO4 0.6, NaNO3 4, inoculation time 8 h, inoculum amount 3%（ψ）, initial pH value 7.5, 37℃, and 180 r/min. In the flask fermentation, the maximum γ-PGA yield reached 44.7 g/L for 24 h, and the highest specific productivity was 1.49 g/（L·h）, being twice of the ultra high molecular weight γ-PGA reported previously. In a 6.6 L fermentor, 32 g/L γ-PGA was obtained for 33 h with a specific productivity of 0.97 g/（L·h）.

关 键 词：地衣芽孢杆菌 Γ-聚谷氨酸 优化 批式发酵 

分 类 号：Q815[生物学—生物工程]