TRAIL联合三氧化二砷诱导骨肉瘤细胞凋亡的实验研究  

Study on the apoptosis of osteosarcoma cells induced by combination of TRAIL and arsenic trioxide

作　　者：黄涛[1] 李旭辉[2] 邹春平[1] 李修成[1] 冯殿鹏[1] 

机构地区：[1]中国医科大学附属第一医院骨科,辽宁沈阳110001 [2]清华大学生命科学学院,北京100084

出　　处：《现代肿瘤医学》2012年第5期899-902,共4页Journal of Modern Oncology

基　　金：辽宁省教育厅高等学校科技计划项目(L2010655);沈阳市科学技术计划项目(1072054-5-00)

摘　　要：目的:研究TRAIL和三氧化二砷共同作用于骨肉瘤OS732细胞对其产生的的杀伤作用,来探寻临床上骨肉瘤的治疗新策略。方法:选取骨肉瘤OS732细胞作为研究对象,将TRAIL及三氧化二砷分别独自或者共同作用于骨肉瘤细胞,对骨肉瘤细胞的杀伤作用通过MTT法来测定,以抑制率的形式体现出来,并在倒置显微镜、荧光显微镜下分别观察肿瘤细胞的形态改变。结果:50ng/ml的TRAIL与2μmol/L三氧化二砷共同作用于骨肉瘤细胞24小时后,肿瘤细胞抑制率为51.32%±4.71%,此作用明显强于单独应用TRAIL(50ng/ml)时的9.85%±0.97%及单独应用三氧化二砷(2μmol/L)时的20.36%±3.84%(P<0.01)。从细胞形态结构的改变上体现出此种杀伤作用是通过诱导细胞凋亡实现的。结论:TRAIL与三氧化二砷可以协同杀伤骨肉瘤细胞,这种杀伤效应是通过促进OS732细胞的凋亡来实现的。Objective：To study the inhibitive effect of combination of TRAIL and As2O3 on the osteosarcoma cell lines and explore the nerw therapy of osteosarcoma.Methods：TRAIL and As2O3 were used to treat the OS732 osteosarcoma cells individually or jointly and observe their effects on inducing apoptosis of osteosarcoma cells.Results：The inhibition rate was 51.32% by MTT essay which was significantly higher than the application of 50ng/ml TRAIL（9.85%） and 2μmol/L As2O3（20.36%）respectively（P0.01）,after twenty-four hours of the application of 50ng/ml TRAIL and 2μmol/L As2O3 jointly on OS732 cells,we observed morphological changes with inverted phase contrast microscope,fluorescence microscope which showed tumor-killing effect of combined application was much stronger than individual application.Conclusion：Combination of TRAIL and As2O3 is obviously conducive to killing effect of osteosarcoma cells which were achieved by inducing apoptosis.

关 键 词：肿瘤坏死因子相关凋亡诱导配体 骨肉瘤 三氧化二砷 

分 类 号：R73-36[医药卫生—肿瘤] R738.1[医药卫生—临床医学]