高糖波动对MG63细胞株护骨素与TRAIL表达的影响  

Effects of high glucose fluctuation on expressions of TRAIL and OPG in MG63 cells

作　　者：周玮 杜云翔 王建国 王加林 

机构地区：[1]解放军82医院内分泌科,江苏淮安223001

出　　处：《东南国防医药》2012年第2期141-144,共4页Military Medical Journal of Southeast China

摘　　要：目的研究波动性葡萄糖和恒定高葡萄糖对MG63细胞株肿瘤坏死因子相关凋亡诱导配体(tumor necrosis factor-related apoptosis-inducing ligand,TRAIL)、护骨素(osteoprotegerin,OPG)和其配体(osteoprotegerin ligand,OPGL)表达的影响,探讨高糖波动在糖尿病性骨质疏松症发病中的作用。方法体外培养的人成骨肉瘤MG63细胞株,随机分为正常对照组(NG组,葡萄糖浓度为5.5 mmol/L);恒定高葡萄糖组(HG组,葡萄糖浓度为33.3 mmol/L);波动性葡萄糖组(FG组,葡萄糖浓度为5.5 mmol/L与葡萄糖浓度为33.3 mmol/L两种培养液,每8 h更换一次),共作用24 h。四甲基偶氮唑蓝(MTT)比色分析法测定细胞增殖,流式细胞仪检测细胞周期及凋亡率。RT-PCR法检测TRAIL、OPG、OPGL mRNA的表达。结果①高糖及高糖波动可抑制MG63细胞的增殖,与HG组相比,FG组的抑制效应更加明显。②高糖及高糖波动可阻滞MG63细胞周期,使G1期细胞比例增加,S期比例减少,诱导细胞凋亡。FG组作用更加明显(P<0.05)。③高糖作用下MG63细胞中TRAIL和OPGL基因表达增加,而OPG基因表达下降,FG组较HG组上述作用更加明显(P<0.05)。结论高糖波动可能导致成骨细胞中TRAIL和OPGL表达增多,OPG的表达减少,抑制成骨细胞增殖,阻滞其细胞周期,诱导细胞凋亡,可能是糖尿病性骨质疏松症的一个重要的发病机制。Objective To observe the regulative effects of glucose at different concentrations on the expressions of tumor necrosis factor-related apoptosis,which inducing ligand（TRAIL）,osteoprotegerin（OPG）,and the ligand of osteoprotegerin（OPGL） in osteosarcoma MG63 cells in order to study the role of high glucose fluctuation in pathogenesis of diabetic osteoporosis.Methods MG63 cells were randomly divided into the following medium containing 5.5 mmol/L glucose（Normal glucose group,NG group）,33.3 mmol/L glucose（High glucose group,HG group） and 5.5/33.3 mmol/L glucose（high glucose fluctuation of different amplitude 5.5/33.3 mmol/L group,FG group） and cultured in vitro for 24 h.The proliferation of MG63 cells was examined by MTT colorimetric analysis.The cell cycle and apoptotic rate of cells were determined by flow cytometry.The expressions of TRAIL,OPG and OPGL mRNA were detected by RT-PCR.Results Both high glucose and high glucose fluctuation inhibited the proliferation of MG63 cells,and the effect of high glucose fluctuation was significantly higher than that in high glucose（P0.05）.Both high glucose and high glucose fluctuation blocked cell cycle,increased the percentage of G1 phase,decreased the percentage of S phase and induced apoptosis.The effect of high glucose fluctuation was obviously stronger than that in high glucose（P0.05）.The mRNA expressions of TRAIL and OPGL in MG63 cells significantly increased and the expression of OPG mRNA decreased under high glucose environment（P0.05）.The mentioned effects were obviously power in FG group than that in HG group.Conclusion High glucose fluctuation could not only inhibit the proliferation,block cell cycle,induce apoptosis,and also lead to the increasing expressions of TRAIL and OPGL,but the decreasing expression of OPG in osteoblasts,which may be one of the key pathogenetic factors of diabetic osteoporosis.

关 键 词：高糖波动 MG63细胞 肿瘤坏死因子相关凋亡诱导配体 

分 类 号：R574[医药卫生—消化系统]