荧光定量聚合酶链反应和流式细胞术检测乳腺癌微转移的研究  

Study on sensitivity of fluorescence quantitative polymerase chain reaction and flow cytometry in detection of breast cancer micrometastasis

作　　者：董秀玲[1] 黄英[1] 佟倩[1] 薛红[1] 高亚杰[2] 

机构地区：[1]大连市第三人民医院肿瘤科,大连116033 [2]大连医科大学附属第一医院,大连116011

出　　处：《中国肿瘤临床与康复》2012年第2期101-104,共4页Chinese Journal of Clinical Oncology and Rehabilitation

基　　金：国家自然科学基金赞助项目(3570479)

摘　　要：目的探讨采用实时荧光定量聚合链反应(FQ-PCR)和流式细胞术(FCM),检测乳腺癌患者外周血中CK-19的表达情况及其临床意义。方法用FQ-PCR和FCM方法检测98例Ⅱ～Ⅲ期乳腺癌患者外周血中CK-19的表达情况,另取同期52例乳腺良性肿瘤手术患者作为对照组。结果采用FQ-PCR术和FCM法检测乳腺癌组患者CK-19的阳性率分别为39.8%(39/98)和28.6%(28/98);对照组未检测到CK-19的阳性表达。两种方法及两组间比较,差异均有统计学意义(P<0.05)。激素受体阳性患者CK-19的阳性率分别为39.6%(21/53)和24.5%(13/53),与阴性患者间差异无统计学意义(P>0.05)。HER-2阳性患者检测CK-19的阳性率分别为42.9%(12/28)和35.7%(10/28),与HER-2阴性患者间差异无统计学意义(P>0.05)。有淋巴结转移者CK-19的阳性率分别为49.1%(28/57)和36.8%(21/57),与无淋巴结转移患者差异有统计学意义(P<0.05)。Ⅲ期CK-19的阳性率分别为55.6%(22/36)和41.7%(15/36),高于Ⅱ期的30.6%(19/62)和21.0%(13/62),差异有统计学意义(P<0.05)。结论 FQ-PCR检测微转移的敏感性高于FCM。CK-19的表达与淋巴结转移和肿瘤分期有关,与激素受体、HER-2表达无关。Objective Detect the expression of cytokeratin 19（CK-19） in peripheral blood of breast cancer patients by fluorescence quantitative polymerase chain reaction（FQ-PCR）and flow cytometry（FCM）,in order to evaluate the sensitivity in detection of micrometastasis,and its clinical significance.MethodsPeripheral blood samples were analyzed in 98 patients with breast cancer（stage Ⅱ-Ⅲ）.52 patients of breast benign tumor were as the control group.FQ-PCR and FCM were used to detect the expression of cytokeratin 19 in peripheral blood.Results The positive rate of CK-19 in breast cancer group was 39.8%（39/98） by FQ-PCR and 28.6%（28/98） by FCM nespectively,while control group turned out to be negative.There were significant difference between the tow groups（P0.05） and the two methods（P0.05）.The positive rate of CK-19 in ER（＋） and /or PR（＋） patients was 39.6%（21/53） by FQ-PCR and 24.5%（13/53）by FCM nespectively.There was no significant difference with positive rate of ER（-） and PR（-） patients（P0.05）.The positive rate of CK-19 in HER2（＋）patients was 42.9%（12/28） by FQ-PCR and 35.7%（10/28） by FCM nespectively.There was no significant difference with positive rate of HER2（-） patients（P0.05）.The positive rate of CK-19 in patients who had metastasis in lymph nodes was 49.1%（28/57）by FQ-PCR and 36.8%（21/57） by FCM nespectively,which had significant difference with patients who didn′t have metastasis in lymph nodes（P0.05）.The positive rate of CK-19 in patients of stage was 55.6%（22/36） by FQ-PCR and 41.7%（15/36） by FCM nespectively.It′s higher than in patients of stage Ⅱ which was 30.6%（19/62）,21%（13/62）.There was significant difference in positive rate of CK-19 between the two stages.（P0.05）.Conclusions The sensitivity of FQ-PCR is higher than FCM in detection of micrometastasis.The expression of CK-19 has no relation to ER,PR and HER-2 status,but has relation to metastasis in lymph nodes and stage.

关 键 词：乳腺肿瘤 肿瘤转移 角蛋白19 聚合酶链反应 流式细胞术 

分 类 号：R737.9[医药卫生—肿瘤] R730.3[医药卫生—临床医学]