顺铂联合5-氟尿嘧啶对肺癌A549细胞生长的影响  

Inhibitory effect of cisplatin combined 5-fluorouracil on the proliferation of non-small cell lung cancer A549 cells

作　　者：潘晋坤[1] 李宝平[1] 张雷[1] 任宏伟[1] 许峰[1] 

机构地区：[1]山西医科大学第二医院肿瘤生物治疗科,山西太原030001

出　　处：《中国现代医生》2012年第13期4-5,10,共3页China Modern Doctor

摘　　要：目的探讨顺铂和5-氟尿嘧啶(5-FU)在体外联合用药对人肺癌A549细胞的生长抑制和促凋亡作用。方法运用四甲基偶氮唑盐(MTT)法检测顺铂和5-FU单独及共同作用48 h后对A549细胞生长的影响,每个药物浓度设6个复孔,实验重复3次,结果取平均值;并用流式细胞术检测两药合用对细胞周期和凋亡的影响。结果 MTT法结果显示顺铂和5-FU联合使用对A549细胞的生长抑制率高于两药单独使用(P<0.01);流式细胞仪检测可见两药合用后晚期凋亡明显增多,S期细胞增多(P<0.01)。结论在体外实验中,顺铂与5-FU联合使用能够更好地抑制肺癌A549细胞的增殖,并可改变细胞周期分布,促进细胞凋亡。Objective To explore the proliferation inhibition and proapoptosis effects of cisplatin combined 5-fluorouracil on human non-small cell lung cancer A549 cells in vitro. Methods The absorbance value was tested by colorimetry through MTF method to evaluate the effect of cisplatin and 5-fluorouracil after 48 hours. Each drug concentration was in six holes and it repeated three times. The influence of cell cycle and apoptosis of cisplatin and 5-fluorouracil was used flow cytometry detection. Results It was proved that cisplatin and 5-fluorouracil could inhibit the proliferation of A549 cells. The inhibition was higher than they alone and it had statistically significant difference （P 〈 0.01）. The cell cycle analysis showed that the percentage of S phase cells increased. The cell apoptosis analysis showed that the percentage of late apoptosis^increased （P 〈 0.01）. Conclusion It can be concluded that cisplatin and 5-fluorouracil could inhibit the proliferation of A549 cells in vitro. This inhibition enhances when cisplatin is combined with 5-fluorouracil.

关 键 词：非小细胞肺癌 顺铂 5-氟尿嘧啶 MTT法 流式细胞术 

分 类 号：R734.2[医药卫生—肿瘤]