出生后小鼠第一磨牙牙胚不同发育阶段的Notch1的表达  

Expression of Notch1 at different developmental stages of tooth germ of first molar of postnatal mouse

作　　者：包志凡[1] 丁振江[1] 陈旭[1] 

机构地区：[1]中国医科大学口腔医学院儿童口腔科,沈阳110002

出　　处：《口腔医学》2012年第4期200-202,共3页Stomatology

基　　金：辽宁省教育厅科学技术研究项目(2009A750)

摘　　要：目的分析出生后小鼠的第一磨牙牙胚发育不同阶段的Notch1的表达,进一步揭示Notch信号在牙齿发育,尤其是牙根发育过程中的作用。方法用Western blot方法检测出生后1 d(PN1)、PN3、PN5、PN7、PN10、PN14和PN21的小鼠下颌第一磨牙牙胚中Notch1的表达,以β-actin作为内参,对阳性条带的强度用NIH ImageJ图像分析软件进行分析,应用SPSS11.0软件进行统计学处理。结果在小鼠PN1和PN3,Notch1表达都较强,在PN5和PN7,Notch1的表达迅速下降,但在PN10,Notch1的表达又开始增强,PN14开始逐渐降低,到PN21达到最低水平,但仍可检测到Notch1的微弱表达。结论Notch1信号在出生后小鼠牙齿的发育过程中继续发挥作用,尤其在牙冠开始钙化,牙根形成的启动等阶段可能发挥重要作用;在牙根发育接近完成时牙髓中仍有Notch1的表达,提示其可能参与牙髓损伤后的修复过程。Objective To analyze the expression of Notch1 in the first molar tooth germ of postnatal mouse at different stages of tooth development and to further indicate the role of Notch signaling in the tooth development,especially in the tooth root development.Methods The expression levels of Notch1 were detected in the first molar tooth germ of the mouse during postnatal stages PN1（1 day）,PN3,PN5,PN7,PN10,PN14,and PN21 by Western blot.β-actin was used for reference.Bands intensities were compared using NIH ImageJ software.The statistical software package SPSS 11.0 was used for the statistical analysis.Results Expression of Notch 1 was strong at PN1 and PN3 stages.In PN5 and PN7,Notch1 expression decreased rapidly,but increased in PN10 and then decreased slightly in PN14,and dropped to its lowest level in PN21 when it could still be detected in a weak fashion.Conclusions Notch1 signaling continued its role in tooth development in mouse postnatal stages.In PN1 and PN3 of mouse,Notch1 signaling might be in active status because the crown of the first molar began its calcification.In PN10,Notch1 signaling was initiated again to was involve in the root formation.Notch1 might play a role in the reparation of dental pulp after completion of root formation since there was still Notch1 expression at that later tooth developmental stage.

关 键 词：NOTCH1 牙胚 牙齿发育 

分 类 号：R349.54[医药卫生—基础医学]