丁苯酞对放射性脑损伤大鼠学习记忆能力及海马MMP-9表达的影响  

Effects of dl-3n-butylphthalide on study and memory abilities and hippocampus expression of MMP-9 in rat model of radiation injuries of brain

作　　者：张谦[1] 刘运林[1] 

机构地区：[1]泰安市中心医院神经内科,泰安271000

出　　处：《中华行为医学与脑科学杂志》2012年第4期318-320,共3页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：基金项目：山东省自然科学基金资助项目（ZR2011HM001）,山东省医药卫生科技发展计划项目（2011HZ102）

摘　　要：目的探讨丁苯酞对放射性脑损伤大鼠学习记忆能力及海马MMP-9表达的影响。方法分组采用直线加速器对Wistar大鼠进行全脑照射，剂量为30Gy。在照射前和照射后120d分别进行Morris水迷宫实验，对大鼠进行学习记忆能力的测试。结果Morris水迷宫试验的结果显示：在学习能力试验中，与模型组相比，丁苯酞大剂量组和丁苯酞小剂量组的跳台逃避潜伏期明显缩短（P〈0．01），分别为（79．61±7．17）s，（43．64±4．72）s，（42．15±4．19）s，错误次数明显减少（P〈0．01），分别为（36．43±6．59）次，（14．58±4．22）次，（13．66±3．91）次；在记忆能力试验中，与模型组相比（17．57±4．29）S，丁苯酞大剂量组和丁苯酞d,N量组原平台象限停留时间明显延长（P〈0．01），分别为（30．09±3．68）s，（31．25±3．17）S，游泳速度明显增快（P〈0．01）。结论丁苯酞可使海马组织MMP-9的表达水平降低，提示丁苯酞对放射性脑损伤的治疗可能是通过改变MMP-9的表达而发挥作用。Objective To study the effects of dl-3n-butylpbthalide on study and memory abilities and hip- pocampus expression of MMP-9 in rat model of radiation injuries of brain. Methods Wistar rats were divided into radiation group radiated by linear accelerator and control group . Morris water test were taken to study the learning and memory ability of rats in each group before irradiation and 120 d after irradiation. Results Morris water maze test results showed that：in the academic test,compared with model group, high-dose buty]phthalide group and low dose butylphthalide group had significantly shorter escape latency（ （79.61 ±7.17） s, （43.64±4.72） s, （42.15 ± 4.19 ） s） and decreased number of errors （ 36.43 ±6.59,14.58 ± 4.22,13.66 ± 3.91 ） （P 〈 0.01 ）. In the memory test, compared with model group, higb-dose butylphthalide group and low dose butylpbthalide group bad signifi- cantly longer the former platform quadrant time respectively （ 30.09 ± 3.68 ） s, （ 31.25 ± 3. 17 ） s vs （ 17.57 ± 4.29 ） s and faster swimming speed respectively （P 〈 0.01 ）. Conclusion Butylphthalide can significantly reduce hippocampal neurons＇expression of MMP-9, which indicates that butylphthalide has curative effect on radiation brain injuries by regulating the expression of MMD-9.

关 键 词：丁苯酞 放射性脑损伤 学习记忆 大鼠 

分 类 号：R-332[医药卫生]