芝麻粕降解菌株的筛选诱变及鉴定  

Screen and Mutation of a Degradation Bacterium for Sesame Meal and Its Molecular Identification

作　　者：娄立起[1] 田璨熙[1] 李磊[1] 谭军[1] 吴永尧[1] 

机构地区：[1]湖南农业大学生物科学技术学院,湖南长沙410128

出　　处：《湖南农业科学》2012年第4期17-19,共3页Hunan Agricultural Sciences

基　　金：湖南省中南烟草实验站项目(08-10Aa02)

摘　　要：从自然界筛选得到一株能够降解芝麻粕粗蛋白的菌株LQ,对其16S rRNA基因序列进行了测定,构建了分子系统发生树,分析了相关细菌相应序列的同源性。结果表明:菌株LQ的16S rRNA基因序列长度为1 447 bp,与芽孢菌属等细菌的16SrRNA基因序列自然聚类,在检索出的芽孢杆菌序列中,该菌株与解淀粉芽孢杆菌的同源性达99%,为解淀粉芽孢杆菌。对菌株LQ进行紫外诱变,其蛋白酶活力587.3 U/mL。A bacteria strain LQ, which can degrade the crude protein of sesame meal, was screened from the nature. In addition, its 16S rRNA gene sequence was determined, its molecular phylogenetic tree was constructed, and its homology with corresponding sequence of related bacteria was analyzed. The results showed that the length of the 16S rRNA gene sequence of LQ was 1 447 bp; natural clustering the 16S rRNA gene sequence of LQ with the 16S rRNA gene sequence of bacteria （such as Bacillus Cohn, etc.）, the results indicated that among the retrieved sequences of Bacillus, the homology between LQ and Bacillus amyloliquefaciens reached 99%, therefore, the LQ is identified as Bacillus amyloliquefaciens. After the LQ strain was mutated by UV mutagenesis, its activity of protease reached 587.3 U/mL.

关 键 词：芝麻粕 诱变 16S RRNA 同源性 

分 类 号：Q933[生物学—微生物学]