丁草胺降解菌P10的分离鉴定及其对植株修复效果的研究  

Isolation,Identification and Plant Remediation Effect of Butachlor Degrading Strain P10

作　　者：彭亚军[1] 程小梅[1] 王俊华[1] 李欣[1] 蔡海林[1] 周小毛[1] 柏连阳[1,2] 

机构地区：[1]湖南农业大学农药研究所,湖南长沙410128 [2]湖南人文科技学院,湖南娄底417000

出　　处：《湖南农业科学》2012年第4期85-87,共3页Hunan Agricultural Sciences

基　　金：南方烟区除草剂药害综合防控关键技术研究与示范(SR0558)

摘　　要：通过富集培养技术从长期受丁草胺污染的污泥中分离出一株丁草胺高效降解细菌,命名为P10,经形态特征、生理生化特征和16S rDNA序列同源性分析,将该菌株鉴定为克雷白氏杆菌属(klebsiella sp.)。P10在30℃,pH 7.0条件下7 d可降解89.6%的丁草胺;在28℃,16 h光照和8 h黑暗的条件下培养10 d,该菌株对药害植株修复效果明显。A strain of butachlor degrading bacterium was isolated from long-term butachlor contaminated soil by enrichment culture technology, which was named as P10. According to morphologic, physiological and biochemical characteristics as well as homology of 16S rDNA sequence, the P10 was identified as an klebsiella sp.. The P10 can degrade 89.6% of butachlor when conditions were 30℃, pH value=7.0, 7 d; when culture of contaminated plant with P10 at 28℃ for 10 d with 16 h of illumination and 8 h of darkness, the bioremediation effect was obvious.

关 键 词：丁草胺 分离鉴定 高效液相色谱 克雷白氏杆菌属 生物修复 

分 类 号：X172[环境科学与工程—环境科学]