p38信号通路在脂多糖诱导的大鼠系膜细胞产生白细胞介素1β中的作用  

The role of p38MAPK in the production of inflammatory cytokines in rat glomerular mesangial cells caused by LPS

作　　者：余学清[1] 李锦华[1] 黄凌虹[1] 陈永雄[1] 顾军[2] 

机构地区：[1]中山医科大学附属第一医院肾内科卫生部肾脏病临床研究重点实验室,广州510080 [2]中山医科大学分子医学研究中心

出　　处：《中华肾脏病杂志》2000年第2期98-101,共4页Chinese Journal of Nephrology

基　　金：国家自然科学基金!39730140;国家自然科学基金!39970704;中山医科大学"211工程"研究基金

摘　　要：目的探讨p38信号通路在肾小球系膜细胞促炎介质白细胞介素1（IL-1）β表达中的作用。方法采用免疫沉淀法、免疫复合物蛋白激酶测定，Westernblotting检测p38信号通路在脂多糖（LPS）诱导的肾小球系膜细胞炎症反应中的活化程度，应用逆转录-聚合酶链反应（RT－PCR）和ELISA观察p38信号通路抑制剂SB203580对肾小球系膜细胞促炎介质IL－1βmRNA和蛋白质表达的影响。结果脂多糖刺激肾小球系膜细胞引起p38信号通路活化，p38信号通路抑制剂SB203580对肾小球系膜细胞促炎介质表达有显著抑制作用。结论p38信号通路在大鼠系膜细胞炎症反应产生炎症介质IL－1β中可能起主要的作用。Objective To investigate the action and mechanism of p38 mitogen-activated protein kinase(p38MAPK) in inducing inflammatory cytokines in rat glomerular mesangial cells caused by LPS. Method Immunoprecipitation, immune complex protein kinase assay and Westem blotting were used to detect p38MAPK in rat glomerular mesangial cells caused by LPS. RT-PCR and EUSA were used to investigate the effect of SB203580 on transcription of IL-1β mRNA and translation of protein in mesangial cells. Results LPS caused activation of p38MAPK at time and dosage dependent manner, and SB203580inhibited not only activation of p38MAPK but also production of proinflammatory cytokines at time and dosage dependent manner. Conclusion p38MAPK may play an important role in production of proinflammatory factors in the rat glomerular mesangial cells.

关 键 词：肾小球系膜细胞 炎症 P38信号通路 脂多糖 IL-1 

分 类 号：R363[医药卫生—病理学]