抗癌活性肽对人肿瘤细胞株增殖的影响及其机理探讨  

ASSESSMENT OF AN ANTI-CANCER BIOACTIVE PEPTIDE ON HUMAN TUMOR CELL AND POSSIBLE MECHANISMS

作　　者：崔宏伟[1] 苏依拉其木格 苏秀兰[1] 

机构地区：[1]内蒙古医学院附属医院临床医学研究中心,内蒙古呼和浩特010050

出　　处：《内蒙古医学院学报》2012年第1期49-55,共7页Acta Academiae Medicinae Neimongol

基　　金：国家自然科学基金(30860327)

摘　　要：目的:探讨由山羊内脏提取制备的抗癌活性肽(Anti-cancer active peptide,ACBP)对人胃癌细胞株BGC-823、MGC-803、人鼻咽癌细胞株CNE增殖的影响及作用机理。方法:应用MTT法和流式细胞术观察不同剂量ACBP在不同时间对体外培养的BGC-823、MGC-803及CNE细胞增殖的影响,并对细胞周期及凋亡进行了检测。结果:MTT结果显示ACBP对BGC-823、MGC-803及CNE的增殖有抑制作用且呈现剂量依赖效应,在ACBP 20ug/mL时,对三种细胞株的抑制率分别达到38.3%、29.79%和42.02%(P<0.01))。流式细胞术检测细胞周期结果显示,3种对照组细胞大部分处于G0/G1期和S期,培养24h后,S期细胞数开始减少,G0/G1期细胞数增加,48h和72h后呈现显著性变化(P<0.01)。流式细胞术检测细胞凋亡结果显示ACBP作用早期,凋亡细胞(AV+/PI-)增多,在48h和72h后更加明显。结论:ACBP对分化不同的胃癌细胞BGC-823、MGC-803及鼻咽癌CNE细胞的增殖有抑制作用,其作用机制可能为诱导细胞凋亡,抑制DNA合成。Objective：To investigating the effects of anti-cancer bioactive peptide（ACBP）obtained from the goat organs on different human tumour cell lines and exploring its possible mechanisms.Methods：Huaman gastric cancer cell lines（BGC-823,MGC-803）and human nasopharyngeal cancer cell lines（CNE）were grown in the media with different doses of ACBP.The effect of ACBP on these cell growth were monitored by MTT assay,flow cytometry（FCM）and apoptotic assay at different times.Results：ACBP could inhibit the proliferation of all three cell lines（38.3%,29.79%and 42.02%respectively at 20g/mL）（P〈0.01）in a dose dependent manner.The FCM showed that the majority of the three control group cells were in G0/G1 phase and S phase,but reduced in number in S phase and increased in G0/G1 phase in ACBP group after 24h（P〈0.01）.The cell apoptosis results also indicated that the number of apoptosis cell（AV＋/PI-）increased in the early stage in ACBP group,became obvious in 48h and 72h.Conclusions：ACBP could inhibit the proliferation of human tumour cell lines.The possible mechanisms are inducing tumor cells apoptosis and suppressing DNA synthesis.

关 键 词：抗癌活性肽 肿瘤细胞系 细胞凋亡 细胞周期 流式细胞术 

分 类 号：R735.7[医药卫生—肿瘤]