种植体周围袋中福赛类杆菌的PCR检测及临床意义  

THE PCR DETECTION AND CLINIC SIGNIFICANCE ABOUT BACTEROIDES FORSYTHUS IN THE PATIENTS WITH PERI-IMPLANTITIS

在线阅读下载全文

作  者:王海涛[1] 隋素琴[1] 白燕[1] 张晓敏[1] 杨慧[1] 曹庆堂[1] 

机构地区:[1]内蒙古医学院第四附属医院口腔科,内蒙古包头014030

出  处:《内蒙古医学院学报》2012年第2期133-138,共6页Acta Academiae Medicinae Neimongol

摘  要:目的:探讨福赛类杆菌在牙周健康者龈沟液中、慢性牙周炎病人牙周袋内、种植体周围炎病人种植周围袋内的含量及其与感染发生的关系。方法:收集58名牙周健康者、60例慢性牙周炎病人、56例慢性种植体周围炎病人龈下菌斑,采用免疫荧光定量16S RNA PCR方法检测福赛类杆菌的含量与分布。结果牙周健康者龈沟内福赛类杆菌的检出率为8.62%,平均Ct值为0.0122±0.0000;慢性牙周炎病人龈下菌斑福赛类杆菌的检出率为81.67%,平均Ct值为0.0356±0.0018;慢性种植体周围炎病人龈下菌斑赛类杆菌的检出率为85.71%,平均Ct值为0.0563±0.0021。结论:种植体周围炎、慢性牙周炎的发生与福赛类杆菌的感染有相关性,种植体周围炎、慢性牙周炎的发病机理可能相一致。Objective:we can provide the basis for evaluation in peri -implantitis diagnosis and treatment through exploring the peri - implant and content of Bacteroides forsythus ( B. f) in pathogenesis and analysing the distribution prevalance peri -implantitis patients,chronic periodontitis patients and periodontally healthy adults. Method: Subgingival plaque samples were collected from 56 peri - implantitis patients,60 chronic periodontitis patients and 58 periodontally healthy adults. Bacteroides forsythus were detected by 16S rRNA polymerase chain reaction. Results:The prevalance of B. f dif- fered significantly in patients with peri - implantitis, patients with chronic periodontitis and periodontal- ly healthy adults (P 〈 0. 001 ). The prevalance of B. f in disease sites of patients with peri - implantitis, chronic periodontitis and periodontally healthy adults was 8.62 %, 81.67 % and85.71% respectively. The Ct of B. f in peri - implantitis, chronic periodontitis and periodontally healthy adults was 0.0122±0. 0000,0. 0356 ± 0. 0018 and 0. 0563± 0. 0021. There is no difference about Ct of P. gingivalis in pa- tients with peri - implantitis and patients with chronic periodontitis ( P 〉 0.05 ). Ct of B. f in peri - im- plantitis is higher than chronic periodontitis ( P 〈 0.05 ). Conclusion: These results indicate a possible association between chronical periodontal disease, chronical peri -implantitis disease and content of B. f, Implant denture need more special maintenance.

关 键 词:福赛类杆菌 种植体周围炎 聚合酶链反应 

分 类 号:R780.2[医药卫生—口腔医学]

 

参考文献:

正在载入数据...

 

二级参考文献:

正在载入数据...

 

耦合文献:

正在载入数据...

 

引证文献:

正在载入数据...

 

二级引证文献:

正在载入数据...

 

同被引文献:

正在载入数据...

 

相关期刊文献:

正在载入数据...

相关的主题
相关的作者对象
相关的机构对象