Identification of Bovine Casein Phosphorylation Using Titanium Dioxide Enrichment in Combination with Nano Electrospray Ionization Tandem Mass Spectrometry  被引量:1

Identification of Bovine Casein Phosphorylation Using Titanium Dioxide Enrichment in Combination with Nano Electrospray Ionization Tandem Mass Spectrometry

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作  者:LI Shan-shan WANG Jia-qi WEI Hong-yang YANG Yong-xin BU Deng-pan ZHANG Le-ying ZHOU Ling-yun 

机构地区:[1]State Key Laboratory of Animal Nutrition,Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,P.R.China

出  处:《Journal of Integrative Agriculture》2012年第3期439-445,共7页农业科学学报(英文版)

基  金:supported by an earmark fund for the National Key Basic Research Program of China(2011CB100805)

摘  要:Protein phosphorylation is an important post-translational modification that regulates milk protein structure and function.The objective of this study was to analyze the presence of phosphorylated casein.Bovine milk proteins were first separated by SDS polyacrylamide gel electrophoresis.After in gels digestion and extraction,phosphorylated peptides were enriched by titanium dioxide and identified by ultra performance liquid chromatography coupled with nano electrospray ionization tandem mass spectrometry.This method ensured the identification of 20 phosphorylated peptides,including 7 phosphorylated forms of α_s1-casein,8 α_s2-casein,and 5 β-casein.Eight phosphorylated sites derived from 3 α_s1-caseins,3 α_s2-caseins,and 2 β-caseins were also identified,and localized on residues Ser^61,Ser^63 and Ser^130 in α_s1-casein;Thr^145,Ser^146 and Ser^158 in α_s2-casein;and Ser^50 and Thr^56 in β-casein.These findings provide valuable information for investigating casein phosphorylation of the bovine milk.Protein phosphorylation is an important post-translational modification that regulates milk protein structure and function.The objective of this study was to analyze the presence of phosphorylated casein.Bovine milk proteins were first separated by SDS polyacrylamide gel electrophoresis.After in gels digestion and extraction,phosphorylated peptides were enriched by titanium dioxide and identified by ultra performance liquid chromatography coupled with nano electrospray ionization tandem mass spectrometry.This method ensured the identification of 20 phosphorylated peptides,including 7 phosphorylated forms of α_s1-casein,8 α_s2-casein,and 5 β-casein.Eight phosphorylated sites derived from 3 α_s1-caseins,3 α_s2-caseins,and 2 β-caseins were also identified,and localized on residues Ser^61,Ser^63 and Ser^130 in α_s1-casein;Thr^145,Ser^146 and Ser^158 in α_s2-casein;and Ser^50 and Thr^56 in β-casein.These findings provide valuable information for investigating casein phosphorylation of the bovine milk.

关 键 词:bovine milk casein PHOSPHORYLATION mass spectrometry titanium dioxide enrichment 

分 类 号:Q503[生物学—生物化学] S311[农业科学—作物栽培与耕作技术]

 

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