Epigenetic reprogramming of embryos derived from sperm frozen at-20℃  

作　　者：CHAO ShiBin LI JianChun JIN XuanJin TANG HaiXun WANG GongXian GAO GuoLan 

机构地区：[1]Center of Reproductive Medicine, the First Affiliated Hospital of Nanchang University, Nanchang 330006, China [2]Department of Obstetrics and Gynecology, Aviation General Hospital of China, Beijing 100101, China

出　　处：《Science China(Life Sciences)》2012年第4期349-357,共9页中国科学（生命科学英文版）

基　　金：supported by the National Basic Research Program of China (Grant No. 2011CB944501)

摘　　要：Cryopreservation of spermatozoa is a strategy that has been used to conserve the sperm of animal species and animal strains that are valuable for biomedical research. A simple method for preserving spermatozoa after application of intracytoplasmic sperm injection （ICSI） is much needed. It has been shown previously that spermatozoa frozen at -20~C can activate oocytes and support full-term embryo development. However, epigenetic reprogramming could be affected by the environment and by the in vitro manipulation of gametes. Here, we investigated embryo epigenetic reprogramming including DNA methylation and histone modification, in embryos derived from sperm preserved at -20~C without cryoprotectants. The results showed that alt- hough both fertilization and embryo developmental competence were decreased, the dynamic epigenetic reprogramming of embryos derived from frozen sperm was similar to the reprogramrning of embryos derived from fresh sperm. The results re- ported in this study indicate that sperm frozen without cryoprotectant is epigenetically safe for ICSI.Cryopreservation of spermatozoa is a strategy that has been used to conserve the sperm of animal species and animal strains that are valuable for biomedical research. A simple method for preserving spermatozoa after application of intracytoplasmic sperm injection (ICSI) is much needed. It has been shown previously that spermatozoa frozen at 20°C can activate oocytes and support full-term embryo development. However, epigenetic reprogramming could be affected by the environment and by the in vitro manipulation of gametes. Here, we investigated embryo epigenetic reprogramming including DNA methylation and histone modification, in embryos derived from sperm preserved at 20°C without cryoprotectants. The results showed that although both fertilization and embryo developmental competence were decreased, the dynamic epigenetic reprogramming of embryos derived from frozen sperm was similar to the reprogramming of embryos derived from fresh sperm. The results reported in this study indicate that sperm frozen without cryoprotectant is epigenetically safe for ICSI.

关 键 词：spermatozoa freezing ICSI EPIGENETIC histone modification DNA methylation 

分 类 号：S965.128[农业科学—水产养殖] S823.36[农业科学—水产科学]