内质网应激预处理对人正常肝细胞缺氧再复氧损伤的保护作用  

Protective Effect of Endoplasmic Reticulum Stress Preconditioning on the HL-7702 Cell Injuried by Hypoxia-Reoxygenation

作　　者：郑志刚[1] 吴楠[1] 周倜[1] 刘栋[1] 陈勇[1] 

机构地区：[1]第四军医大学西京医院肝胆外科,陕西西安710032

出　　处：《现代生物医学进展》2012年第8期1426-1429,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金(81070363)

摘　　要：目的:研究内质网应激预处理对人肝细胞缺氧复氧损伤的保护作用。方法:将培养的人肝细胞分为4组:正常对照(C)组、细胞缺氧复氧损伤(H/R)组、内质网应激(ER)组、内质网应激预处理(ERP+H/R)组。收集各组细胞,以流式细胞仪检测细胞凋亡,Western-bloting及RT-PCR检测内质网应激特异蛋白GRP78表达水平,并通过透射电镜观察各组细胞超微结构改变。结果:ERP+H/R组细胞凋亡率明显低于H/R组(P<0.05),ER及ERP+H/R组GRP78蛋白表达明显高于H/R组(P<0.05)。结论:内质网应激预处理对肝细胞缺氧复氧损伤具有明显的保护作用,内质网应激特异性蛋白GRP78可能在肝细胞缺氧复氧损伤中作为一种关键性的保护蛋白出现。Objective： To study the protective effects on the endoplasmic reticulum stress preconditioning on human hepatic cell hypoxia-reoxygenation injury.Methods： Cultured human liver cells into four groups,namely normal control（C） group,hypoxia-reoxygenation（H / R） group,endoplasmic reticulum stress（ER） group,endoplasmic reticulum stress preconditioning（ERP ＋ H / R） group.The cells were collected to detect apoptosis by flow cytometry,Western-bloting and RT-PCR detection of endoplasmic reticulum stress protein GRP78-specific expression,and ultrastructural changes of cells were observed by transmission electron microscopy.Results： ERP ＋ H / R cell apoptosis was significantly lower than H / R group（P〈0.05）,ER and ERP ＋ H / R group GRP78 protein expression was significantly higher than the H / R group（P〈0.05）.Conclusion： Endoplasmic reticulum stress preconditioning on hypoxia-reoxygenation injury of liver cells has a significant protective effect.Endoplasmic reticulum stress-specific protein GRP78 is a key protective protein in liver cells injured by hypoxia-reoxygenation.

关 键 词：内质网应激 预处理 人正常肝细胞 缺氧再复氧损伤 

分 类 号：R364.12[医药卫生—病理学]