小肠结肠炎耶尔森氏菌和高碘对SD大鼠的影响  

Effect of Yersinia enterocolitia and excess iodine to SD rats

作　　者：张冬冬[1] 张军[1] 

机构地区：[1]滨州医学院附属医院内分泌科,滨州256603

出　　处：《滨州医学院学报》2012年第1期16-18,共3页Journal of Binzhou Medical University

摘　　要：目的观察小肠结肠炎耶尔森氏菌和高碘对SD大鼠血清中甲状腺相关激素和IL-10、IL-12变化的影响。方法用小肠结肠炎耶尔森氏菌尾静脉注射大鼠,同时给予不同剂量高碘饮食。将SD大鼠随机分为正常组、模型组、高碘组(低、中、高剂量组),3个月时放射免疫法检测各组大鼠血清游离三碘甲腺原氨酸(FT3)、游离甲状腺素(FT4)、促甲状腺激素(TSH)的含量,ELISA法检测TSH受体刺激性抗体(TSAb)及IL-10、IL-12含量。结果模型组和高碘组IL-10、IL-12明显升高,高碘组与模型组间及高碘组不同剂量间差异有统计学意义;高碘组甲状腺激素与模型组间及高碘组不同剂量间无统计学意义。结论小肠结肠炎耶尔森氏菌可致大鼠IL-10、IL-12水平升高,过量碘独立于甲状腺功能之外加重了Graves病大鼠的免疫功能紊乱。Objective To observe the effect of Yersinia enterocolitia and excess iodine on the changes of the thyroid related hormones and IL-10,IL-12 in SD rats.Methods Rats were immuned with Yersinia enterocolitia through the tail vein,while given different doses of excess iodine diet.SD rats were randomly divided into three groups： control group,model group and excessive iodine group（low,middle,high dose）.Three months later,radio-immunity method was used to examine the contents of FT3,FT4 and TSH in rats＇ serum,while ELISA method to TSAb,IL-10,IL-12.Results Serum level of IL-10,IL-12 increased significantly in model group and excess iodine group;Significant difference was found about IL-10,IL-12 between excess iodine and model group also among different doses of excess iodine,while the serum level of thyroid hormones was not found significant difference.Conclusion Yersinia enterocolitia can improve the level of IL-10 and IL-12 in rat serum;excess iodine aggravate the immune dysfunction of the Graves＇ Disease rats independent of the function of thyroid.

关 键 词：小肠结肠炎耶尔森氏菌 碘 GRAVES病 甲状腺激素 TSAb、IL-10、IL-12 

分 类 号：R581.1[医药卫生—内分泌]