黏虫蜜源植物花蜜中游离氨基酸的测定与分析  

Determination and Analysis of Free Amino Acids in Nectar Source of Armyworm Moth

作　　者：刘保玲[1] 王高平[1] 胡京枝[2] 

机构地区：[1]河南农业大学植物保护学院,河南郑州450002 [2]河南省农业科学院农业质量标准与检测技术研究中心,河南郑州450002

出　　处：《河南农业科学》2012年第4期93-96,共4页Journal of Henan Agricultural Sciences

基　　金：河南省教育厅自然科学研究资助计划项目(2010A210013)

摘　　要：为探讨黏虫成虫与迁飞中转地蜜源植物的营养关系,利用氨基酸分析仪测定了其蜜源植物油菜和紫云英花蜜中游离氨基酸的种类和含量。结果显示,油菜和紫云英花蜜中都含17种氨基酸,油菜花蜜中游离氨基酸的总含量是紫云英花蜜的2.47倍。紫云英花蜜与油菜花蜜中各游离氨基酸的含量不同。油菜花蜜中除胱氨酸、蛋氨酸、苯丙氨酸、赖氨酸、精氨酸外,其他游离氨基酸的含量均比紫云英花蜜中相应的氨基酸高,其中丙氨酸、酪氨酸、丝氨酸、甘氨酸、天冬氨酸的含量分别是紫云英花蜜的14.36倍、11.35倍、5.70倍、4.69倍、4.11倍。研究还发现,紫云英蜂蜜中氨基酸总量是紫云英花蜜中氨基酸总量的4.29倍,油菜花蜜比油菜蜂蜜多含了胱氨酸和亮氨酸。因此,不能用油菜蜂蜜、紫云英蜂蜜代替油菜花蜜、紫云英花蜜来研究紫云英和油菜花蜜对黏虫的营养效应。Milk vetch（Astragalus sinicus）and rape（Brassica napus） are primary nectar plants for oriental armyworm（Mythimna separata）.Milk vetch was reported as a key factor resulting in severe outbreaks of oriental armyworm in China.This study collected nectar of milk vetch and rape by the capillary method,and then determined the type and content of free amino acids by the amino acid analyzer.Results showed that there were 17 amino acids in both rape nectar and milk vetch nectar.The total free amino acid content in rape nectar was 2.47 times as much as the content in milk vetch nectar.The content of each free amino acid was different between milk vetch nectar and rape nectar： The content in rape nectar was higher than that of the corresponding amino acid in milk vetch nectar except Cys,Met,Phe,Lys,Arg;The contents of Ala,Tys,Ser,Gly,and Asp in rape nectar were 14.36,11.35,5.70,4.69,and 4.11 times as much as those in milk vetch nectar.The total free amino acid content in milk vetch honey was 4.29 times as much as that in milk vetch nectar,and Cys and Leu were existed in rape nectar but not in rape honey.So milk vetch honey or rape honey can not be used instead of milk vetch nectar or rape nectar to study the nutritional effects on armyworm moth.

关 键 词：黏虫 蜜源植物 紫云英 油菜 花蜜 蜂蜜 游离氨基酸 

分 类 号：S897[农业科学—特种经济动物饲养]