氯化甲基汞-L-半胱氨酸共轭物诱导恶性脑胶质瘤凋亡的体外研究  

Effect of methylmercury chloride-L-cysteine conjugate on apoptosis of malignant glioma cells in vitro

作　　者：刘秀花[1] 费秉元[2] 袁长吉[1] 李志超[1] 杨四宝[2] 丛丹[2] 林芳竹[2] 陶慧超[2] 

机构地区：[1]吉林大学第一医院肿瘤中心,吉林长春130021 [2]吉林大学白求恩医学院,医学试验班吉林长春130021

出　　处：《中国实验诊断学》2012年第4期579-581,共3页Chinese Journal of Laboratory Diagnosis

基　　金：大学生创新实验计划项目2010A71102

摘　　要：目的探讨氯化甲基汞(methylmercury chloride,MMC)-L-半胱氨酸(L-cys)共轭物体外诱导恶性脑胶质瘤U251细胞凋亡并抑制其增殖的作用。方法采用四甲基偶氮唑蓝(MTT)比色法测定不同剂量的MMC-L-cys共轭物分别作用24、48、72h后对U251胶质瘤细胞增殖的影响,并与三氧化二砷(ATO)做平行对照研究。流式细胞仪用异硫氰酸荧光素标记的膜联蛋白V(Annexin V-FITC)和碘化丙啶(PI)双染检测凋亡情况。结果 MMC-L-cys共轭物抑制U251胶质瘤细胞的生长和增殖,与正常对照组相比差异显著(P<0.05),其抑制作用强于ATO组(P<0.05),抑制作用呈剂量、时间依赖性;U251胶质瘤经不同浓度的MMC-L-cys共轭物作用后,24h其凋亡率显著高于对照组(P<0.05),诱导凋亡作用具有剂量依赖关系。结论 MMC-L-cys共轭物能诱导胶质瘤细胞凋亡、抑制其增殖,呈时间剂量依赖性,为胶质瘤的治疗提供实验依据。Objective To study the effect of methylmercury chloride-L-cysteine conjugate on apoptosis and proliferation inhibitory of U251 glioma cells in vitro.Methods MTT assay was performed to evaluate the proliferation inhibitory effect of MMC-L-cys conjugate with different concentrations on cultured U251 glioma cells for 24、48、72 h.Flow cytometry was used to assess the effect of MMC-L-cys conjugate treatment on cell apoptosis in U251 glioma cells.Results MMC-L-cys conjugate could inhibit the proliferation of cultured U251 glioma cells in vitro,the viabilities were significantly lower than those in control group or ATO group（P〈0.05）,the inhibitory effect was in a does-time-dependent manner.When U251 glioma cells were treated with MMC-L-cys for 24 h,the apoptosis rates were significantly higher than those in control group（P〈0.05）,the effect was in a dose-dependent manner.Conclusion MMC-L-cys conjugate could induce apoptosis and inhibit proliferantion of cultured U251 glioma cells in vitro,the effect was in a dose and time dependent manner.

关 键 词：氯化甲基汞 L-半胱氨酸 神经胶质瘤 凋亡 

分 类 号：R739.4[医药卫生—肿瘤]