膀胱移行细胞癌组织基因的P14ARF、DAPK、RARβ甲基化变异分析  

Methylation status of DAPK,RARβ,P14ARF gene in Bladder Transitional Cell Carcinoma

作　　者：王凯臣[1] 孙然[2] 倪培民[2] 王亮[2] 孔祥波[1] 丛宪玲[2] 

机构地区：[1]吉林大学中日联谊医院泌尿外科,吉林长春130033 [2]吉林大学中日联谊医院组织标本库,吉林长春130033

出　　处：《中国实验诊断学》2012年第4期647-649,共3页Chinese Journal of Laboratory Diagnosis

基　　金：吉林省科技发展计划项目资助(20080739)

摘　　要：目的通过检测新鲜冰冻膀胱癌组织中P14ARF、DAPK、RARβ基因的启动子CpG岛基因的甲基化状态,探讨P14ARF、DAPK、RARβ在膀胱癌发生过程中的作用及诊断意义。方法采用甲基化特异性PCR技术检测48例手术切除的膀胱癌组织和其中13例相应癌旁组织标本、17例非肿瘤患者正常膀胱组织中DAPK、RARβ、P14ARF基因的甲基化情况。结果 48例膀胱癌组织中发现P14ARF基因异常甲基化13例(27.0%),DAPK基因异常甲基化13例(27.0%);RARβ基因异常甲基化44例(91.6%);17例腺性膀胱炎组织中发现P14ARF基因异常甲基化0例,DAPK基因异常甲基化2例(11.7%);RARβ基因异常甲基化3例(17.6%);13例正常膀胱组织中,仅有2例RARβ基因异常甲基化,P14ARF基因、RARβ基因异常甲基化膀胱癌组织与腺性膀胱炎组织差异有统计学意义(P<0.01;P<0.05)。结论 P14ARF、DAPK、RARβ基因异常甲基化与膀胱癌发病密切相关,RARβ基因可作为正常膀胱组织与癌组织鉴别诊断的标志物。Objective To investigate the P14ARF,DAPK,RARβ promoter methylation status and prognosis of bladder carcinoma.Methods Methylation status of P14ARF,DAPK,RARβ gene promoter in 48 specimens of bladder carcinoma,17 cystitis glandularis tissues and 13 normal bladder tissues were detected by methylation-specific PCR.Results The methylation rate of P14ARF,DAPK,RARβ were in bladder carcinoma 27.0%,27.0%,91.6% respectively;The methylation rate of P14ARF,DAPK,RARβ were in cystitis glandularis tissues 0%,11.7%,17.6% respectively;The methylation rate of RARβ were in 13 normal bladder tissues 15.3%,respectively.Between the bladder carcinoma group and the cystitis glandularis group the methylation rates of P14ARF and RARβ were statistically significant differences（P=0.016;P=0.001）.Conclusion The methylation status of DAPK,P14ARF RARβ was related closely to the development of bladder carcinoma,the methylation status of RARβ may become a new potential biomarker of prognosis in Bladder Transitional Cell Carcinoma.

关 键 词：膀胱移行细胞癌 P14ARF DAPK RARβ 

分 类 号：R737.14[医药卫生—肿瘤]