220例患者TCT标本高危HPV E6/E7 mRNA检测及分析  

Detection and analysis of high-risk HPV E6/E7 mRNA in TCT specimens of 220 patients

作　　者：嵇海舟[1] 何诚[2] 刘桐宇[1] 谢榕[1] 陈刚[2] 郑雄伟[2] 林玉珍[1] 杨琳[1] 

机构地区：[1]福建省肿瘤医院妇瘤科,福州350014 [2]福建省肿瘤医院病理科,福州350014

出　　处：《福建医药杂志》2012年第2期62-64,共3页Fujian Medical Journal

基　　金：福建省卫生厅青年基金资助项目(2009-2-29)

摘　　要：目的利用膜式液基薄层细胞学检测(liquid-based thin layer cytology test,TCT)标本检测高危人乳头瘤病毒(human papilloma virus,HPV)E6/E7mRNA并分析其在宫颈癌筛查中的应用。方法检测220例TCT标本中的HPVE6/E7mRNA,并对HPV E6/E7mRNA表达的总体分布情况及在不同细胞学级别中的分布情况行统计学分析。结果不同细胞诊断级别E6/E7拷贝数的异差异有统计学意义(单因素ANOVA检验)。随着宫颈病变程度增加,HPV E6/E7mRNA的表达率相应增加。结论 TCT标本可用于行高危HPV E6/E7mRNA检测;检测HPV E6/E7mRNA对宫颈癌的筛查有临床意义。Objective To evaluate the application of HR HPV E6/E7 rnRNA in cervical cancer screening by detecting the transcripts in TCT samples. Methods HR-HPV E6/E7 mRNA of 220 samples containing five high grades （ASCUS/ASC-H/ LSIL/HSIL/cancer） were detected and the data were statistically analyzed. Results The level of statistical significance of the variance in all five grades of samples was determined using Student-Newman-Keuls test at the significance level of 0.05. The ex- pression levels of HPV E6/E7 mRNA transcripts were found to be correlated with the development of severe epithelial cervical dysplasia. Conclusion The residual TCT samples might be used in the detection of high risk HPV E6/E7 mRNA. HPV E6 and E7 mRNA test could be a useful tool for cervical cancer screening.

关 键 词：人乳头瘤病毒 MRNA DNA 膜式液基薄层细胞学检查 E6/E7 

分 类 号：R737.33[医药卫生—肿瘤]