三氧化二砷抑制苯并(a)芘诱导的HeLa细胞细胞色素P450 1A1的表达  

Inhibitory Effect of Arsenic Trioxide on Expression of CYP1A1 in Benzo(a)pyrene-induced HeLa Cells

作　　者：耿建[1,2] 石玉荣[1,2] 王慧[2] 

机构地区：[1]安徽省蚌埠市蚌埠医学院生物化学与分子生物学教研室,蚌埠233000 [2]安徽省蚌埠市蚌埠医学院检验诊断学实验中心,蚌埠233000

出　　处：《华中科技大学学报（医学版）》2012年第2期161-164,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：安徽省高等学校青年教师科研基金资助项目(No.2008jq1107);安徽省高等学校省级自然科学研究项目(No.KJ2012Z248)

摘　　要：目的观察不同浓度三氧化二砷(As2O3)对苯并(a)芘[B(a)P]诱导的子宫颈癌HeLa细胞的增殖及细胞色素P450 1A1(CYP1A1)表达的影响。方法用7.5μmol/L B(a)P分别与不同浓度的As2O3共同作用于HeLa细胞,MTT法检测As2O3对HeLa细胞增殖的影响,Western blot和实时荧光定量PCR分别检测CYP1A1的蛋白表达和mRNA表达,荧光素标记检测CYP1A1活性。结果 MTT法结果显示,随着As2O3浓度增加,各实验组吸光度值逐渐减少,与对照组相比,差异均有统计学意义(P<0.05);As2O3下调B(a)P诱导的HeLa细胞CYP1A1的mRNA和蛋白质的表达,抑制作用显著(P<0.05)。CYP1A1活性检测结果显示,随着As2O3浓度增加,HeLa细胞CYP1A1酶活力逐渐降低,各实验组与对照组差异均有统计学意义(P<0.05)。结论 As2O3能抑制B(a)P诱导的HeLa细胞增殖,抑制CYP1A1的表达并降低CYP1A1酶活性且呈现剂量依赖性。Objective To observe the effect of the different concentrations of As2Os on the proliferation of HeLa cells and the expression of CYP1A1 in benzo（a）pyrene（BaP）-induced in HeLa cells. Methods HeLa cells were cultured with 7.5 μmol/L B（a）P plus different concentrations of As2 Os. MTT assay was used to measure cell proliferation, reaLtime fluorescence quantitative PCR and Western blot methods were used to detect the expression of CYP1A1 mRNA and protein,ad fluorescence labeling methods were used to determine CYP1A1 activity. Results MTT assay showed that absorbance（A）values in experi- mental group were decreased gradually as compared with control group （P〈 0.05）. As2Os could inhibit the expression of CYP1A1 protein and mRNA in B（a）P-induced HeLa cells significantly（P〈0.05）. CYP1A1 enzyme activity was decreased with the increased concentration of As2 Os in HeLa cells, and there was significant difference between experimental group and control group（P〈0.05）. Conclusion As203 can inhibit the cell proliferation and the expression of CYP1A1 in HeLa cells induced by B（a）P,and decrease the enzyme activity of CYP1A1 in a dose dependent manner.

关 键 词：三氧化二砷 苯并(A)芘 细胞色素P450 1A1 HELA细胞 

分 类 号：R737.33[医药卫生—肿瘤]