链球菌表面烯醇化酶重组蛋白多克隆抗体的制备  

PREPARATION OF RECOMBINANT STREPTOCOCCAL SURFACE ENOLASE POLYCLONAL ANTIBODY

作　　者：许丽萍[1,2,3] 白文成[2,3] 刘杨[4] 韩润林[2,3] 

机构地区：[1]内蒙古医学院基础医学院,呼和浩特010059 [2]内蒙古农业大学兽医学院,呼和浩特010018 [3]血浆脂蛋白免疫学研究中心,呼和浩特010018 [4]内蒙古农业大学生物工程学院,呼和浩特010018

出　　处：《内蒙古农业大学学报（自然科学版）》2012年第1期20-23,共4页Journal of Inner Mongolia Agricultural University(Natural Science Edition)

基　　金：国家自然科学基金(30860019)资助

摘　　要：制备M6型GAS表面烯醇化酶抗体。皮下多点注射GAS表面烯醇化酶重组蛋白(rSEN)免疫家兔,采用亲和层析纯化免疫血清,通过SDS-PAGE、ELISA、Western-Blot等方法检测抗体的特异性,并初步用制备的抗体检测了M6血清型GAS表面烯醇化酶的表达。制备的抗体蛋白纯度较高,检测的特异性较强,用制备的抗体可以检测到活体菌表面的烯醇化酶。成功制备并纯化得到了GAS表面烯醇化酶多克隆抗体。To prepare anti - rSEN antibody and to detect the express of surface enolase of GAS. Rabbit was administered three times with rSEN subcutaneously by mudi - dot, pured immune serum by affinity chromatograph and detected the specification of anti - rSEN polyelonal antibody with SDS - PAGE, Western - Blot and ELISA. The expression of surface enolase of serotype M6 of GAS also was determined with anti - rSEN antibody. Prepared anti - rSEN polyclonal antibody was profuse and specification is obvious. The purified anti - rSEN antibody can detect the express of surface enolase of GAS. Anti - rSEN polyclonal antibody was succusfully prepared and purified.

关 键 词：A群链球菌 表面烯醇化酶 多克隆抗体 

分 类 号：R914[医药卫生—药物化学]