Gene expression by simian virus 40 large Tantigen-induced medulloblastomas in mice  

作　　者：Xiaoluan Wei Jie Feng Yinghe Hu 

机构地区：[1]Shanghai Engineering Research Center of Molecular Therapeutics and New Drug Development, Key Laboratory of Brain Functional Genomics, MOE ＆ STCSM, East China Normal University, Shanghai 200062, China [2]Shanghai Laboratory Animal Research Center, Shanghai 200032, China

出　　处：《Neural Regeneration Research》2012年第12期932-937,共6页中国神经再生研究（英文版）

基　　金：supported by the National Natural Science Foundation of China,No.31000574;the Fundamental Research Fund for the Central Universities,No.78210042;the Development Program of China during the 10~(th) Five-Year Plan Period,No.2001BA70113

摘　　要：Signaling pathways known to have components with mutations in human medulloblastoma include sonic hedgehog, Wnt/beta-catenin and insulin-like growth factor. Microarray analysis was applied to examine the gene expression changes in medulloblastomas of pTet-on/pTRE-SV40Tag transgenic mice. Altogether, 14 112 genes were detectable, including 152 genes with significantly different expression levels. These genes were associated with immunity, the cell cycle, signal transduction, cytoskeleton and metabolism. To further confirm the microarray data, real-time polymerase chain reactions were used to examine the expression changes of genes related to sonic hedgehog, Wnt/beta-catenin and insulin-like growth factor signal pathways. Immunohistochemistry detected insulin receptor substrate-1 in the nuclei of brain tumor tissue cells from pTet-on/pTRE-SV40Tag transgenic mice, suggesting that SV40 large T antigen may activate the insulin-like growth factor signal pathway to promote tumorigenesis.Signaling pathways known to have components with mutations in human medulloblastoma include sonic hedgehog, Wnt/beta-catenin and insulin-like growth factor. Microarray analysis was applied to examine the gene expression changes in medulloblastomas of pTet-on/pTRE-SV40Tag transgenic mice. Altogether, 14 112 genes were detectable, including 152 genes with significantly different expression levels. These genes were associated with immunity, the cell cycle, signal transduction, cytoskeleton and metabolism. To further confirm the microarray data, real-time polymerase chain reactions were used to examine the expression changes of genes related to sonic hedgehog, Wnt/beta-catenin and insulin-like growth factor signal pathways. Immunohistochemistry detected insulin receptor substrate-1 in the nuclei of brain tumor tissue cells from pTet-on/pTRE-SV40Tag transgenic mice, suggesting that SV40 large T antigen may activate the insulin-like growth factor signal pathway to promote tumorigenesis.

关 键 词：SV40 large T antigen MEDULLOBLASTOMA cDNA microarray real-time polymerase chain reaction insulin-like growth factor 

分 类 号：Q78[生物学—分子生物学] S852.65[农业科学—基础兽医学]