3,4-methylenedioxyamphetamine upregulates p75 neurotrophin receptor protein expression in the rat brain  

作　　者：Chaomin Wang Zugui Peng Weihong Kuang Hanyu Zheng Jiang Long Xue Wang 

机构地区：[1]Mental Health Center, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China

出　　处：《Neural Regeneration Research》2012年第12期955-959,共5页中国神经再生研究（英文版）

基　　金：supported by the National Natural Science Foundation of China (MDMA Neurotoxicity and Mechanism),No.C090302

摘　　要：The p75 neurotrophin receptor, which is a member of the tumor necrosis factor receptor superfamil facilitates apoptosis during development and following central nervous system injury. Previous studies have shown that programmed cell death is likely involved in the neurotoxic effects of 3, 4-methylenedioxy-N-methylamphetamine （MDMA）, because MDMA induces apoptosis of immortalized neurons through regulation of proteins belonging to the Bcl-2 family. In the present study, intrapedtoneal injection of different doses of MDMA （20, 50, and 100 mg/kg） induced significant behavioral changes, such as increased excitability, increased activity, and irritability in rats. Moreover, changes exhibited dose-dependent adaptation. Following MDMA injection in rat brain tissue, the number of apoptotic cells dose-dependently increased and p75 neurotrophin receptor expression significantly increased in the prefrontal cortex, cerebellum, and hippocampus. These findings confirmed that MDMA induced neuronal apoptosis, and results suggested that this effect was related by upregulated protein expression of the p75 neurotrophin receptor.The p75 neurotrophin receptor, which is a member of the tumor necrosis factor receptor superfamil facilitates apoptosis during development and following central nervous system injury. Previous studies have shown that programmed cell death is likely involved in the neurotoxic effects of 3, 4-methylenedioxy-N-methylamphetamine （MDMA）, because MDMA induces apoptosis of immortalized neurons through regulation of proteins belonging to the Bcl-2 family. In the present study, intrapedtoneal injection of different doses of MDMA （20, 50, and 100 mg/kg） induced significant behavioral changes, such as increased excitability, increased activity, and irritability in rats. Moreover, changes exhibited dose-dependent adaptation. Following MDMA injection in rat brain tissue, the number of apoptotic cells dose-dependently increased and p75 neurotrophin receptor expression significantly increased in the prefrontal cortex, cerebellum, and hippocampus. These findings confirmed that MDMA induced neuronal apoptosis, and results suggested that this effect was related by upregulated protein expression of the p75 neurotrophin receptor.

关 键 词：3  4-methylenedioxyamphetamine APOPTOSIS CYTOTOXICITY neural regeneration p75 neurotrophin receptor 

分 类 号：Q593.2[生物学—生物化学] S476.13[农业科学—农业昆虫与害虫防治]