干扰素-β对SAH致心肺损害的影响  

Effects of interferon-β on cardiopulmonary function after subarachnoid hemorrhage

作　　者：孟凡良[1] 孟雷[2] 彭洪海[2] 史建国[2] 刘殿玮[2] 姜勇[2] 

机构地区：[1]山东大学医学院,济南250012 [2]山东大学附属济南市中心医院神经外科,济南250013

出　　处：《山东大学学报（医学版）》2012年第5期32-35,共4页Journal of Shandong University:Health Sciences

基　　金：济南市科技局科技攻关计划基金资助项目(061038-2);济南市科技局发展计划基金资助项目(200807032)

摘　　要：目的研究蛛网膜下腔出血(SAH)后血管内皮细胞生长因子C(VEGF-C)在心、肺的表达及重组人干扰素-β(INF-β)对心肺功能的保护作用。方法采用小脑延髓池注血法建立蛛网膜下腔出血模型,将实验动物随机分为正常对照组、假手术组、SAH未治疗组和SAH治疗组,采用免疫组织化学SP法观察VEGF-C在心脏、肺脏中的表达并测定积分光密度值(IOD)。结果与假手术组相比,SAH未治疗组和SAH治疗组,心脏和肺脏中VEGF-C的表达在24、48、72 h时均明显升高(P均<0.05);SAH治疗组24、48、72 h时,VEGF-C在心脏和肺脏中的表达均低于SAH未治疗组(P均<0.05)。结论 SAH引起的全身炎症反应导致心、肺缺血,使VEGF-C的表达升高,INF-β可抑制心、肺等脏器的炎症反应并改善组织缺血。Objective To study expression of vascular endothelial growth factor-c（VEGF-C） in hearts and lungs after subarachnoid hemorrhage（SAH） and to evaluate the protection of interferon-β（INF-β）.Methods The rat SAH model was established.The rats were randomly divided into the control group,the sham operation group,the SAH group with therapy and the SAH group without therapy.Expressions of VEGF-C in the hearts and lungs were detected.Results VEGF-C expressions in hearts and lungs in the SAH group with therapy and the SAH group without therapy were higher than the sham operation group.VEGF-C expression in hearts and lungs in the SAH group without therapy was lower than the SAH group with therapy.Conclusion SAH leads to cardiopulmonary ischemia through systemic inflammatory response.The INF-β can protect the organs by restraining the systemic inflammatory response.

关 键 词：蛛网膜下腔出血 血管内皮生长因子-C 干扰素Β 大鼠 

分 类 号：R743.35[医药卫生—神经病学与精神病学]