运用Identifiler试剂盒对硅珠法提取石蜡包埋组织DNA质量的评估  

Using Identifiler Kit evaluates silica extracting DNA from paraffin-embedded tissue

作　　者：林旭[1] 王建文[1] 尹雅莎[2] 谢素梅[1] 冀强[1] 

机构地区：[1]南京医科大学法医学系,江苏南京210029 [2]南京医科大学数学计算机教研室,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2012年第4期577-581,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省教育厅科学基金资助(05KJD310140)

摘　　要：目的:运用硅珠法从石蜡包埋的组织中提出DNA,Identifiler试剂盒评估DNA质量。方法:南京医科大学第一附属医院病理科2009～2011年石蜡包块49例,运用硅珠法提取石蜡包埋组织DNA,通过紫外分光光度计测定DNA纯度和Identifiler试剂盒PCR后3130型毛细管电泳仪分析提取DNA片段的完整性。结果:经紫外分光光度计测定胃癌组织D(260 nm)/D(280nm)均值为1.84±0.02,肺癌组织为1.85±0.02,甲状腺癌组织为1.82±0.02,3组间比较P>0.05,因此硅珠法提取石蜡包埋组织DNA不会因组织差异而影响提取效果;Identifiler试剂盒PCR后毛细管电泳显示其提取效果,46例(93%)得出完整的16个基因座STR峰型。结论:硅珠法可应用于多种组织的DNA提取,并保证提取DNA样品的纯度和完整度。Objective：To extract DNA from paraffin-embedded tissue by silica-extraction.Methods：The DNA samples were obtained via silica-extraction method from 49 cases of paraffin-embedded tumor tissues from Department of Pathology in the First Affiliated Hospital of NJMU.The 16 STR gene loci of PCR-DNA fragments were identified with Identifiler Kit.The purity of DNA extracted was analyzed by ultraviolet spectrophotometer.Results：The mean D（260 nm）/D（280 nm） of DNA extracted from gastric carcinoma was 1.84 ± 0.02,from pulmonary carcinoma is 1.85 ± 0.02,from thyroid carcinoma was 1.82 ± 0.02.There was no significant difference in D（260 nm）/D（280 nm） among three carcinoma type groups（P 0.05）.The 46/49 cases（93%） showed the full peaks of all the 16 STR gene loci of PCR-DNA fragment.Conclusion：Silica-extraction may be used to various kinds of paraffin-embedded tissues and extract DNA with high purity and high integrity.

关 键 词：石蜡包埋组织 硅珠法 Identifiler试剂盒 DNA 

分 类 号：R361.2[医药卫生—病理学]