抑癌基因p16^(INK4)真核表达转移载体的构建  

Construction of eukaryotic transfer vector of tumor suppressor gene p16^( INK4)

作　　者：朱长军[1] 张利宁[2] 马春红[2] 曹英林[2] 赛宁 

机构地区：[1]泰山医学院免疫学教研室,山东泰安271000 [2]山东医科大学微生物免疫学教研室,山东济南250012 [3]山东省泰山疗养院,山东泰安271000

出　　处：《泰山医学院学报》2000年第1期4-6,共3页Journal of Taishan Medical College

基　　金：卫生部科研基金资助项目!(4 13653)

摘　　要：目的　构建可应用于昆虫杆状病毒表达系统的含抑癌基因p16 INK4cDNA的重组转移载体。方法　采用定向克隆方法将抑癌基因p16 INK4cDNA全长插入转移载体质粒pSXIVVI+ X3,并经斑点杂交 ,PCR及酶切鉴定重组质粒。结果　经三种鉴定方法证实p16 INK4cDNA片段成功地插入转移载体pSXIVVI+ X3。结论　重组质粒pSXIVVI+ X3 p16构建成功 ,为真核表达抑癌基因p16 INK4奠定了基础。Objective: To obtain the eukaryotic expression transfer vector containing the tumor suppressor gene p16 INK4 cDNA. Methods:By directional cloning method, p16 INK4 cDNA fragment was cloned into the transfer vector pSXIVVI +X 3 and the recombinant plasmid DNA was identified with dot blot hybridization, PCR, and enzyme digestion. Results: p16 INK4 cDNA fragment had been cloned into the transfer vector pSXIVVI +X 3 successfully. Conclusion: The recombinant plasmid pSXIVVI +X 3 p16 is constructed successfully, which lays a good basis for expressing p16 INK4 gene with insect bacularvirus expression system.

关 键 词：抑癌基因 P16^INK4 肿瘤 转移载体 真核表达 

分 类 号：R73-3[医药卫生—肿瘤]