大规模测序研究CD_(34)^+造血干/祖细胞基因表达谱  

Exploration of gene expression profiles of CD_(34)^+ hematopoietic stem/progenitor cells based on large scale sequencing

作　　者：吴济生[1] 张庆华[1] 叶珉[1] 吴昕彦[1] 周隽[1] 傅刚 黄秋花[1] 顾健[1] 包其郁[2] 余亚平 沈宇 徐淑华[1] 茅矛 陈竺[1] 

机构地区：[1]上海第二医科大学附属瑞金医院 [2]温州医学院

出　　处：《中华血液学杂志》2000年第5期229-233,共5页Chinese Journal of Hematology

基　　金：国家 8 63高科技项目 !( 10 2 10 0 1 0 2 ) ;上海市科委及上海血研所胡应洲基金

摘　　要：目的　建立大规模测序方法 ,并用于造血干 祖细胞 (HSPC)基因表达谱的初步识别。方法　从脐血中分离CD34+ 细胞 ,构建cDNA文库 ,对其进行大规模表达序列标签 (EST)测序 ,用生物信息学的方法进行结果分析。结果　在获得的 986 6条EST中 ,有意义序列归并为 2 0 6 0个连续克隆 ,其中10 5 4个为已知基因 ,10 0 6个为至今尚未被公共数据库公布的新基因片段。 10 5 4个已知基因根据功能分为八个大类 :①造血相关的 73个 ;②染色体结构及细胞分裂相关的 91个 ;③细胞信号传导相关的111个 ;④细胞结构 运动相关的 48个 ;⑤细胞和机体防御相关的 41个 ;⑥基因表达 (转录、翻译及加工 )相关的 2 6 5个 ;⑦代谢相关的 192个 ;⑧未分类的 2 33个。结论　获得了HSPC表达的 10 5 4个已知基因和 10 0 6个新基因片段构成的初步基因表达谱 。Objective To set up a large scale sequencing system and explore the gene expression profiles of CD 34 + hematopoietic stem/progenitor cells (HSPCs). Methods CD 34 + cells were isolated from umbilical cord blood and subjected to cDNA library construction. A primary profile of gene expression in HSPCs was emerged by EST sequencing and bioinformatics analyzing. Results Among 9?866 ESTs thus obtained, 7?476 meaningful ESTs were clustered into 2?060 unique sequence species (USSs), representing 1?054 known gene species and 1?006 unknown gene fragments. The 1054 known genes were divided into 8 categories: ①hematopoiesis associated:73, ②chromatin structure and cell division/apoptosis:91, ③signal transduction and receptors:111, ④cell structure/mobility:48, ⑤cell/organism defense/homeostasis:41;⑥Gene expression (transcription, translation and modification):265, ⑦metabolism:192;and ⑧unclassified:233. Conclusion A gene expression profile including 1?054 known genes and 1006 new gene fragments of HSPCs was primarily obtained, which may lay a basis for the further study on the molecular mechanism of hematopoiesis regulation and provide candidates for new gene cloning.

关 键 词：造血干细胞 序列分析 基因表达 计算生物学 

分 类 号：Q343[生物学—遗传学]