激发型抗CD_(40)单克隆抗体对CD_(40)^+B细胞恶性肿瘤的生长抑制和促凋亡作用  

Anti-CD_(40) McAb induces CD_(40)^+ malignant B cell proliferation arrest and apoptosis

作　　者：周照华[1] 施勤[1] 王江方[1] 潘建忠 张学光[1] 

机构地区：[1]苏州医学院免疫教研室,215007

出　　处：《中华血液学杂志》2000年第5期237-239,共3页Chinese Journal of Hematology

基　　金：杰出人才基金! ( 3 962 5 0 2 4) ;国家自然科学基金青年基金! ( 3 960 0 0 5 6)

摘　　要：目的　研究CD40 抗原在恶性B淋巴细胞肿瘤中的表达及其CD40 抗原激发所致的生物学效应。方法　将激发型CD40 单克隆抗体 (单抗 ) 5C11加入肿瘤细胞的体外培养体系 ,采用细胞计数、流式细胞仪分析等方法分析单抗 5C11对不同肿瘤细胞株的作用。结果　 5C11能引起CD40 表达强阳性的多发性骨髓瘤 (MM)细胞XG2发生同型聚集 ,抑制其生长并介导细胞凋亡 ;5C11可引起CD40+ 的恶性B淋巴瘤细胞株Daudi发生同型聚集 ,抑制其生长并使细胞发生G2 M期阻滞 ,但并不介导细胞凋亡。结论　激发型CD40 单抗通过诱导恶性B淋巴瘤细胞的凋亡或使G2 M期细胞阻滞 。Objective To study the expression of CD 40 molecule and the biological effects mediated by CD 40 molecules on malignant B cells. Methods Agonistic anti human CD 40 monoclonal antibody (5C11) was added to the cell culture system. Cell counting, PI staining, Annexin V staining and flow cytometric analysis were used to study the biological effects of 5C11 on malignant B cell lines. Results 5C11 induced homotypic aggregation, proliferation arrest and apoptosis in the multiple myeloma cell line XG2 highly expressed CD 40 . 5C11 induced B lymphoma cell line Daudi homotypic aggregation and proliferation arrest but no apoptosis. Conclusion 5C11 can inhibit proliferation of malignant B cells by inducing apoptosis or arresting the cells at G 2/M interphase.

关 键 词：CD40抗原 单克隆抗体 多发性骨髓瘤 细胞凋亡 

分 类 号：R73-3[医药卫生—肿瘤]