受体介导TNFα基因转移的实验研究  

Experimental Study on Human Tumor Necrosis Factor α Gene sDelivery via Receptor-mediated Endocytosis

作　　者：陶箭[1] 张国弛[1] 丁健青[1] 张希衡[1] 李彪如[1] 童善庆[1] 

机构地区：[1]上海第二医科大学微生物教研室,上海200025

出　　处：《上海第二医科大学学报》2000年第1期32-34,共3页Acta Universitatis Medicinalis Secondae Shanghai

基　　金：上海市高教局科学技术发展基金!95B07

摘　　要：目的检测受体介导基因转移人肿瘤坏死因子α基因的靶向性和表达效率。方法人转铁蛋白同多聚赖氨酸共价交联，盐键合成交联蛋白和外源基因质粒的超分子复合物，对表达转铁蛋白受体的肿瘤细胞株进行β－半乳糖苷酶和人肿瘤环死因子α基因转移。结果检测到外源基因表达；转染人肿瘤坏死因子α基因后细胞上清液检测到一过性人肿瘤坏死因子α活性升高。结论受体介导基因转移具有良好的靶向性，但表达效率低。Objective To investigate the target and expression efficiency of receptor -mediated TNFα gene delivery. Methods A molecular conjugate vector containing human transferrin was formed and complexed to plasmid pSVβ-gal or pSVk3-TNFαs. The complex was introduced into tumor cell lines which presented different quantity human transferrin receptor. Results The cells could secrete TNFα transiently after being transfected, which could be verified by the L929 cell-killed test and ELISA, as well as a series of molecular biologic methods. Conclusion More special target and lower efficiency were found in receptor-mediated gene transfer than in liposome mediated gene transfer.

关 键 词：转铁蛋白 实验研究 基因转移 肿瘤坏死因子Α 

分 类 号：R446.61[医药卫生—诊断学]