^(131)I-Herceptin对卵巢癌细胞的体外作用  

In vitro radioimmunotherapy of ovarian cancer with ^(131)I-Herceptin

作　　者：熊敏超[1] 石怡珍[2] 胡建铭[3] 刘增礼[2] 朱本兴 

机构地区：[1]武汉大学医学院鄂州市第一医院放射科,鄂州436000 [2]苏州大学附属第二医院核医学科,苏州215004 [3]苏州大学附属第一医院妇产科,苏州215006 [4]江苏省放射医学与防护重点实验室,苏州215004

出　　处：《现代妇产科进展》2012年第4期263-266,270,共5页Progress in Obstetrics and Gynecology

基　　金：高校省级重点实验室开放课题(No:KJS0928);苏州市科技发展计划(社会发展-应用基础研究)(No:SYSD2010119)

摘　　要：目的:研究^(131)I标记抗HER-2/neu单克隆抗体Herceptin(^(131)I-Herceptin)体外对高表达HER-2/neu卵巢癌细胞的生物学作用,探讨将其用于卵巢癌放射免疫治疗的可行性。方法:(1)流式细胞仪检测人卵巢癌SKOV3和HO8910细胞株HER-2/neu的表达。(2)Iodogen法^(131)I标记Herceptin,测定其标记率、放化纯度、稳定性及免疫活性。(3)MTT比色法检测^(131)I-Herceptin对人卵巢癌细胞株SKOV3和HO8910的抑制作用。(4)流式细胞法检测SKOV3在^(131)I-Herceptin作用下细胞周期改变和凋亡情况。结果:(1)SK-OV3细胞株HER-2/neu表达率为92.67%;HO8910表达率为9.59%。(2)^(131)I-Herceptin标记率为89.8%,放化纯度为98.4%,24h后仍大于80%,标记物免疫活性较好。(3)131I-Her-ceptin对SKOV3细胞株的抑制作用明显高于HO8910(P<0.001),且明显高于^(131)I组﹑Her-ceptin组以及^(131)I+Herceptin组(P<0.01)。(4)流式细胞检测^(131)I-Herceptin组SKOV3细胞的凋亡率明显高于^(131)I+Herceptin、Herceptin和^(131)I组(P<0.05),各干预组均检测到细胞周期阻滞,^(131)I-Herceptin组G0-G1期细胞比例明显高于其它三组(P<0.05)。结论:^(131)I-Herceptin对高表达HER-2/neu的人卵巢癌细胞SKOV3有明显的抑制和杀伤作用。Objective：To investigate the biologic effectiveness of ^（131）I-labeled Herceptin antibody（^（131）I-Herceptin）against HER-2/neu on human ovarin cancer cell strain with high HER-2/neu expression in vitro,and to assess its feasibility of radioimmunotherapy in ovarian cancer.Methods：The expression of HER-2/neu antigen in ovarian cancer cell lines SKOV3 and HO8910 was detected by flow cytometer（FCM）.^（131）I-Herceptin was prepared by using Iodogen method.The labeling efficiency,radiochemical purity,stability and immunocompetence were measured.The inhibition effect of ^（131）I-Herceptin on SKOV3 and HO8910 cell lines was evaluated by MTT assay.The cell cycle changes and cell apoptotic rate of the intervention group and control group on SKOV3 were evaluated by FCM.Results：The positive ratios of HER-2/neu expression were 92.67% and 9.59% on SKOV3 cells and HO8910 cells,respectively.The labeling efficiency and radiochemical purity of ^（131）I-Herceptin were 89.8%and 98.4%,respectively.The labeling was stable and showed good immunocompetence.MTT assay indicated the inhibition effect of ^（131）I-Herceptin on SKOV3 cells was significantly higher than that of on HO8910 cells（P0.001）and was also significantly higher than that of ^（131）I,Herceptin and ^（131）I＋Herceptin groups,respectively（P0.01）.By flow cytometer assay,the apoptosis rate and cycle blockage of ^（131）I-Herceptin group were conspicuously higher than those of ^（131）I group,Herceptin group and ^（131）I＋Herceptin group（P0.05）.Conclusions：^（131）I-Herceptin can effectively inhibit and kill the human ovarian cancer cell SKOV3 with high HER-2/neu expression in vitro.

关 键 词：卵巢肿瘤 受体 表皮生长因子 抗体 单克隆 碘放射性同位素 同位素标记 

分 类 号：R737.31[医药卫生—肿瘤]