60Coγ射线照射人胶质瘤细胞U251对糖基转移酶β3GnT8及多聚乳糖胺糖链的影响  

Effect of 60Co γ-ray on the Expression of β3GnT8 and Polylactosamine in Human Glioma Cell Line U251

作　　者：胡水君[1] 姜智[1] 徐岚[1] 邹士涛[1] 吴士良[1] 

机构地区：[1]苏州大学医学部生物化学与分子生物学系,江苏苏州215123

出　　处：《中国血液流变学杂志》2012年第1期9-11,34,共4页Chinese Journal of Hemorheology

基　　金：国家自然科学基金面上项目（31170772）;国防科工基金基础科研项目（B3820110002）

摘　　要：目的 研究60Coγ射线照射胶质瘤细胞U251对β3GnT8及多聚乳糖胺糖链的影响,为治疗恶性肿瘤和研究高效、低毒的辐射防护剂奠定实验基础.方法 采用0Gy、1Gy、5Gy和8Gy剂量的60Coγ射线辐照胶质瘤细胞U251,Western blotting检测60Coγ射线对β3GnT8蛋白水平表达的影响;流式细胞术分析60Coγ射线对多聚乳糖胺糖链表达水平的影响.结果 随着60Coγ射线辐照剂量的增加,β3GnT8蛋白和细胞表面多聚乳糖胺糖链表达水平逐渐降低.结论 60Coγ射线照射胶质瘤细胞U251对β3GnT8和多聚乳糖胺糖链表达均有影响,为研究高效、低毒的辐射防护剂奠定一些实验基础.Objective To provide some experimental evidence for curing malignant turnout and researching highly active and low mammalian toxicity radioprotectant,and to study the effect of ^60Co γ-ray on the expression of β 3GnT8 and polylactosamine in human glioma cell line U251.Methods Using 0Gy, 1Gy,5Gy and 8Gy dose of ^60Co γ-ray to irradiate human glioma cell line U251,using western blotting to measure the β 3GnT8 protein expression level,and using flow cytometry to detect the effect of ^60Co γ-ray on the expression of polylactosamine. Results The expression of β 3GnT8 and polylactosamine decreased gradually as the dose of ^60Co γ-ray increased. Conclusion ^60Co γ-ray affected the expression of β 3GnT8 and polylactosamine in human glioma cell line U251. Providing some experimental evidence for researching highly active and low mammalian toxicity radioprotectant.

关 键 词：Γ射线 β3葡萄糖基转移酶 多聚乳糖胺 

分 类 号：R73-3[医药卫生—肿瘤]