小儿肾母细胞瘤中PRL-3的表达及其临床意义  

The expression and clinical significance of PRL-3 in Wilms tumor in children

作　　者：谭萍[1] 李旭良[1] 

机构地区：[1]重庆医科大学附属儿童医院泌尿外科,儿童发育疾病研究省部共建教育部重点实验室,儿科学重庆市重点实验室,重庆市儿童发育重大疾病诊治与预防国际科技合作基地,400014

出　　处：《中华小儿外科杂志》2012年第5期352-356,共5页Chinese Journal of Pediatric Surgery

摘　　要：目的探讨促肝细胞再生磷酸酶-3（PRL3）在小儿肾母细胞瘤、瘤旁组织中的表达，及其与肾母细胞瘤临床分期、病理类型、复发及化疗的关系。方法用逆转录聚合酶链反应法、免疫组化S-P法从基因和蛋白水平检测39例肾母细胞瘤及30例瘤旁组织中PRL-3的表达，并对其与临床分期、病理类型、复发及化疗等进行相关性分析。结果本组共检测肾母细胞瘤39例，其中30例为新鲜标本，9例为病理科存档石蜡标本；新鲜瘤旁组织30例。肿瘤复发者再次切除获得新鲜复发瘤标本3例。检测结果：PRL-3mRNA在肾母细胞瘤组织中的表达明显高于瘤旁组织（χ2=5．16，P〈0．05），且随临床分期的增高其表达逐渐上升，经Spearman相关分析PRL-3mRNA表达与临床分期呈正相关（rs=0．857，P〈0．05）；肿瘤组织恶性程度越高阳性表达率越高（P2tail=0．0049〈0．05）；复发的肿瘤组织及其原发瘤均为阳性表达；术前经过化疗的组织表达明显低于未经化疗者（Pz。-=0．0348〈0．05）；在蛋白水平的检测也得到了与上述一致的结果。结论PRL-3基因和蛋白在肾母细胞瘤中明显表达，且与肾母细胞瘤的临床分期、恶性程度及是否复发密切相关，提示PRL-3mRNA和蛋白检测不但有助于对肾母细胞瘤临床分期、恶性程度的判断及复发可能性的预测，还可为术后治疗方案的制定提供有力的依据。Objective To investigate the expression of phosphatase of regenerating liver-3（PRL- 3）in Wilms tumor and adjacent kidney tissue and to study the relationship between PRL-3 expression and the clinical stage,pathology grading,recurrence,chemotherapy of Wilms tumor. Methods Reverse transcription polymerase chain reaction（RT-PCR） and Immunohistochemistry S-P were used to meas- ure the expression of PRL-3 in 39 nephroblastoma specimens and 30 controls, which were obtained from adjacent kidney tissues. Itscorrelation with clinical stage, pathological type, recurrence and chem- otherapy was analyzed. Results A total of 39 Wilms tumor specimens（30 fresh specimens and 9 paraf- fin specimens） and 30 fresh adjacent kidney tissues were obtained. 3 more recurrent tumor tissues were also obtained. The positive rate of PRL-3 mRNA and protein in Wilms Tumors was 60. 0% （18/ 30） and 61.5o//oo （24/39）respectively, while that in the adjacent kidney tissues was 13.3%（4/30）, 16. 7% （5/30）. The expression level was significantly different between Wilms Tumors and adjacent kidney tissues （X2 = 5.16,5.34,P〈0. 05）. The positive expression rate of PRL-3 mRNA and protein in stage I,stage Ⅲ ,stageⅢ ,stage Ⅳ of Wilms Tumors was 14. 3% （1/7） ,57. 1% （4/7） ,72. 7% （8/ 11） ,100% （5/5） ; 12. 5% （1/8）, 50. 0% （4/8）, 75. 0% （12/16）, 100% （7/7）, respectively. Spearman correlation analysis showed that the expression of PRL-3 mRNA and protein was positively correlated with the clinical stage（ r＇s =0. 857,0. 839, P%0.05）. The degree of malignancy in tumor tissues alsocorrelated with the positive expression rate of PRL-3 mRNA. 100% （5/5） expression was seen in tumors with the most severe form, while 52. 0% （13/25） was seen in the favorable type. The trend was similar for protein expression： 100% （5/5） for severe form and 55.9% （19/34） for favorable form. The expression of PRL-3 mRNA and protein was significantly different between different degree of malignan

关 键 词：促肝细胞再生磷酸酶-3 肾母细胞瘤 逆转录聚合酶链反应 免疫组织化学 

分 类 号：R737[医药卫生—肿瘤]