ATPR对ECA-109、PANC-1、HeLa细胞增殖及分化的影响  

Effects of a new retinoic acid derivative ATPR on the proliferation and differentiation of ECA-109,PANC-1 and HeLa tumor cells

作　　者：吴菲[1] 陈飞虎[1] 洪凡青[1] 王新群[1] 汪渊[2] 

机构地区：[1]安徽医科大学药学院基础与临床药理教研室,安徽合肥230032 [2]安徽医科大学分子生物学实验室省部共建教育部和安徽省重要遗传病基因资源利用重点实验室,安徽合肥230032

出　　处：《中国癌症杂志》2012年第4期257-263,共7页China Oncology

基　　金：“重大新药创制”科技重大专项(No:2011ZX09401-021)

摘　　要：背景与目的:由于全反式维甲酸(all-trans retinoic acid,ATRA)治疗急性早幼粒细胞白血病(acute promyelocytic leukemia,APL)存在一些不良反应,使得其进一步的临床应用受到了限制。目前,寻找具有更好疗效的维甲酸衍生物已成为研究热点。该研究旨在观察新型维甲酸衍生物4-氨基-2-三氟甲基苯基维甲酸酯(4-amino-2-trifluoromethyl-phenyl retinate,ATPR)对食管癌细胞株ECA-109、胰腺癌细胞株PANC-1、宫颈癌细胞株HeLa生长和分化的影响。方法:采用MTT法检测细胞的生长抑制率;流式细胞仪测定细胞周期的变化;酶联免疫吸符实验(ELISA)检测食管癌细胞分化指标鳞状上皮癌相关抗原SCC-Ag活性、胰腺癌细胞标志物CA199和宫颈癌细胞标志物CA125水平。结果:ATPR能够抑制肿瘤细胞增殖;并使G0/G1期细胞表达量增加,S期细胞表达量减少,细胞阻滞在G0/G1期;ECA-109中SCC-Ag活性下降,PANC-1中CA199和HeLa中CA125水平下降。结论:ATPR对上述3种实体瘤细胞株具有不同程度的诱导分化作用。Background and purpose： Due to some adverse reactions occurs when acute promyelocytic leukemia （APL） treated with all-trans retinoic acid （ATRA）, which limited its further clinical application. At present, looking for a better efficacy of retinoic acid derivatives has become a hot topic. Therefore our study aimed to find the effects of 4-amino-2-trifluoromethyl-phenyl retinate （ATPR） on the proliferation and differentiation of human esophageal cancer cells ECA-109, human pancreatic cancer cells PANC-I and human cervical cancer cells HeLa. Methods： Solid tumor cells were cultured in vitro and treated with ATPR of different concentrations. The proliferation was studied with MTT test. The cell cycle was analyzed by flow cytometry. SCC-Ag, CA199 and CA125 were measured by ELISA. Results： The growth of solid tumor cells treated with ATPR was inhibited in a dose-dependent manner; G0/G1 phase cells were significantly increased while S phase cells were decreased with the elevation of ATPR concentration, Cell cycle progression was blocked in the G0/G1 phase; SCC-Ag of ECA-109 suppressant; CA199 of PANC-1 and CA125 of HeLa decreased. Conclusion： ATPR could induce differentiation of solid tumor cells in different degrees.

关 键 词：4-氨基-2-三氟甲基苯基维甲酸酯 维甲酸衍生物 细胞增殖 细胞分化 

分 类 号：R73-361[医药卫生—肿瘤]