弓形虫SAG1基因在蜥蜴利什曼原虫中的表达  

Expression of SAG1 gene from Toxoplasma gondii in Leishmania tarentolae

作　　者：许越[1] 杨欢欢[1,2] 魏峰[1] 商立民[2] 刘全[2] 

机构地区：[1]吉林农业大学生命科学学院,吉林长春130118 [2]中国人民解放军军事医学科学院,军事兽医研究所,吉林省人兽共患病预防与控制重点实验室,吉林长春130062

出　　处：《中国兽医学报》2012年第5期696-700,共5页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31072127);国家科技支撑计划资助项目(2010BAD04B01)

摘　　要：利用PCR扩增技术从弓形虫RH株全基因组中扩增出弓形虫抗原基因SAG1,将其克隆入真核表达载体质粒pLEXSY-neo2,构建真核表达穿梭质粒pLEXSY-neo2-SAG1。利用电穿孔转染技术将外源基因表达盒转入蜥蜴利什曼原虫,经G418筛选阳性克隆。表达的外源重组蛋白经SDS-PAGE和Western blot进行鉴定,证明SAG1重组蛋白在蜥蜴利什曼原虫中得到表达。In this study,the antigen gene SAG1 of Toxoplasma gondii（T.gondii） was amplified from T.gondii RH strain genome,and cloned into the eukaryotic expression vector pLEXSY-neo2 to construct eukaryotic expression vector pLEXSY-neo2-SAG1,which was transfected by electroporation into the Leishmania tarentolae genome,and positive clones were screened by G418.The expressed proteins were identified by SDS-PAGE and western blot,which was proved to be expressed in the Leishmania tarentolae.

关 键 词：弓形虫 蜥蜴利什曼原虫 SAG1基因 电穿孔转染 表达 

分 类 号：S852.7[农业科学—基础兽医学]