脑卒中患者血浆HDL亚类组成及与血压的相关性  

Study on the Distribution of Plasma HDL Subclasses and the Relationship with the Blood Pressure in Stroke Patients

作　　者：丁岚[1] 田英[1] 乔新惠[1] 王小鸥[2,3] 陈浩[2,3] 陈武哲[1] 陈志军[1] 龙石银[1] 

机构地区：[1]南华大学生物化学与分子生物学教研室 [2]南华大学 [3]南华大学附属第一医院,湖南省衡阳市421001

出　　处：《中国动脉硬化杂志》2012年第5期455-459,共5页Chinese Journal of Arteriosclerosis

基　　金：国家自然科学基金项目(30800474);湖南省科技厅项目(2010SK3037);湖南省教育厅重点项目(11A104);湖南省研究生创新项目(CX2010B385)

摘　　要：目的研究脑卒中患者高密度脂蛋白(high density lipoprotein,HDL)亚类组成及相对百分含量的变化,并探讨脑卒中患者血压与HDL亚类组成的关系。方法采用双向电泳-免疫印迹检测法分析了79例正常对照和98例脑卒中患者HDL亚类组成及相对百分含量。结果脑梗死组preβ1-HDL(P<0.05或P<0.001)、HDL3c(P<0.001)水平显著增加,而HDL2a(P<0.05)及HDL2b(P<0.05或P<0.001)水平显著减少。脑出血组preβ1-HDL、HDL3c含量显著增加(P<0.05),而HDL2b含量显著减少(P<0.001)。受试者按收缩压水平分组发现,随着收缩压水平逐渐升高,小颗粒的preβ1-HDL、HDL3c及HDL3a水平均有升高趋势,而大颗粒的HDL2b水平有降低趋势。与正常组比较,收缩压高组(组Ⅳ:160 mmHg≤收缩压<180 mmHg)及收缩压极高组(组V:收缩压≥180 mmHg)preβ1-HDL、HDL3c含量均显著升高(P<0.05),而HDL2b含量显著降低(P<0.05)。且收缩压水平与preβ1-HDL、HDL3c水平呈正相关(r=0.235,r=0.250),而与HDL2b水平呈负相关(r=-0.228)。结论脑卒中患者HDL颗粒直径呈变小趋势,并且随着收缩压水平升高,其HDL颗粒的变小程度更加明显。Aim To study the distribution of plasma HDL subclasses in stroke patients and the relationship be- tween the levels of the blood pressure and the contents of plasma HDL subclasses in stroke patients. Methods The contents of HDL subclasses in 79 healthy controls and 98 stroke subjects were determined by two-dimensional gel electro- phoresis associated with immunodetection method. Results In contrast to heahhy controls, the contents of preβ1-HDL and HDL3o increased significantly and that of HDL2a and HDL2b were decreased significantly in the cerebral infarction pa- tients （P 〈 0.05）. The levels of preβ1 -HDL and HDL3o were significantly higher and HDL2b level was significantly lower （ P 〈0. 001 ） in cerebral hemorrhage subjects. With the levels of systolic pressure increaing, the contents of preβ1-HDL, HDL3c and HDL3a increased and that of HDL2b decreased. When the pressure was greater than 160 mmHg, the contents of preβ1-HDL and HDL3cwere significantly higher than healthy controls （P 〈0. 05）, and the contents of HDL2b were signifi- candy lower than healthy controls （ P 〈 0. 05 ）. Systolic pressure was positively correlated with preβ1 -HDL and HDL3a （ r = 0. 235 and 0. 250 respectively） and negatively correlated with HDL2b （ r = - 0. 228 respectively）. Conclusions The particle size of HDL in stroke subjects showed a shift toward smaller size in stroke subjects, and the tendency was relat- ed with the increase of systolic pressure levels.

关 键 词：HDL亚类 脑卒中 血压 双向电泳-免疫印迹检测法 

分 类 号：R363[医药卫生—病理学]