悬沙胁迫下三疣梭子蟹仔蟹的RNA/DNA比率和DNA损伤  

RNA/DNA Ratio and DNA Damage of Marsupenaeus japonicus Juvenile Crab Stressed by Suspended Solids

作　　者：李云[1] 沈盎绿[1] 

机构地区：[1]中国水产科学研究院东海水产研究所,上海200090

出　　处：《应用与环境生物学报》2012年第2期251-255,共5页Chinese Journal of Applied and Environmental Biology

基　　金：国家重点基础研究发展规划项目(No.2010CB429005);中央级公益性科研院所基本科研业务费专项资金(中国水产科学研究院东海水产研究所)项目(No.2008M13)资助~~

摘　　要：为探讨悬沙对仔蟹的影响,以三疣梭子蟹(Portunus trituberculatus)仔蟹为受试生物,研究不同悬沙浓度(0、50、100、500、1 000和5 000 mg L-1)下I期仔蟹的成活率、RNA/DNA比率和DNA损伤情况.结果表明,低浓度悬沙(≤500 mg L-1)对仔蟹无明显影响,高浓度悬沙(≥1000 mg L-1)对仔蟹的成活率几乎无影响,但会延迟仔蟹蜕壳变态的时间,降低RNA/DNA比率,导致彗星细胞的拖尾率、尾长和尾矩显著上升.可见,RNA/DNA比率和DNA损伤指标能够有效评估悬沙对仔蟹的影响.Effects of suspended solids（SS） on the survival rate,RNA/DNA ratio and DNA damage of Marsupenaeus japonicus juvenile crab were investigated in this study.The results indicated that the juvenile crabs were not sensitive to the lower concentration SS（≤ 500 mg L-1）.Furthermore,the survival ratio of juvenile crabs was not significantly different in the higher concentration SS groups（≥ 1 000 mg L-1）.But the RNA/DNA ratio decreased,and the DNA damage parameters as comet cell rate,tail length and tail moment increased dramatically in the high concentration SS groups.Our results suggested that the parameters of RNA/DNA ratio and DNA damage were the effective biomarkers for assessing the effects of SS on the juvenile crab.

关 键 词：悬沙 仔蟹 成活率 RNA/DNA比率 DNA损伤 

分 类 号：X174[环境科学与工程—环境科学]