不同血清型沙眼衣原体小鼠生殖道感染模型的建立  

Establishment of a mouse model for genital tract infection with different serovars of Cldamydia trachomatis

作　　者：杨斌[1] 周细玉[1] 郑和平[1] 薛耀华[1] 

机构地区：[1]广东省皮肤性病防治中心,广州510500

出　　处：《中华皮肤科杂志》2012年第5期325-328,共4页Chinese Journal of Dermatology

摘　　要：目的建立不同血清型沙眼衣原体小鼠生殖道感染模型，为研究型别与毒力的关系奠定基础。方法将6周龄BALB／c雌性小鼠分为4组，实验组经孕酮处理后接种4．0×107沙眼衣原体，孕酮对照组接种McCoy细胞培养液，无孕酮对照组直接接种4．0×10，沙眼衣原体，以及单纯小鼠的空白对照。分别于接种后4d起观察小鼠外阴变化，阴道分泌物细胞培养、直接免疫荧光法和实时荧光PCR检测沙眼衣原体。结果实验组接种沙眼衣原体E、F、J和K型，4d后小鼠生殖道表现出轻微的感染迹象，7d后阴道分泌物培养、免疫荧光和PCR检测均检出沙眼衣原体，而对照组为阴性。感染持续时间以K型最长（21d，1／11只阳性），其次为J型（17d，1／11只阳性）、F型（14d，5／11只阳性）和E型（14d，2／11只阳性）。结论适龄小鼠经孕酮处理后，接种一定量的沙眼衣原体，可建立不同血清型沙眼衣原体感染模型。Objective To establish a mouse model for genital tract infection with different serovars of Chlamydia trachomatis （Ct）, and to provide a basis for understanding the relationship between Ct serovars and virulence. Methods Six-week-old female BALB/c mice were divided into 4 groups： study group （pretreated with progesterone and inoculated with 4.0× 10^7 inclusion-forming units （IFU） of Ct serovar E, F, J and K, respectively）, progesterone-control group （pretroated with progesterone and inoculated with culture medium of McCoy cells）, non progesterone-control group （inoculated with 4.0 ×10^7 IFU of Ct serovar F）, blank control group receiving no treatment. At 4 and 7 days after the inoculation, the appearance of vulva was observed, vaginal swab specimens were obtained and subjected to cell culture, direct immunofluorescence assay and real-time fluorescence-based PCR for the detection of Ct. Results A slight iIfflammation of the genital tract was observed in the mice 4 days after the inoculation with Ct serovar E, F, J or K in the study group. Cell culture, direct immunofluorescence assay and real-time fluorescence-based PCR all confirmed that Ct was present in vaginal specimens from the study group, but absent from the control groups. The longest duration of infection was 21 days in 1 out of 11 mice inoculated with Ct serovar K, followed by 17 days in 1 out of 11 mice inoculated with Ct serovar J, 14 days in 5 out of 11 mice with Ct serovar F and in 2 out of ! 1 mice with Ct serovar K. Conclusion The model for genital tract infection with different serovars of Ct can be established in mice at a right age by pretreatment with progesterone and inoculation with a certain amount of Ct.

关 键 词：衣原体 沙眼 小鼠 模型 动物 

分 类 号：R-332[医药卫生]