C3d在大疱性类天疱疮患者皮损组织中的表达  

Detection of C3d expression in bullous oemphiguld

作　　者：郭坤[1] 耿松梅[1] 

机构地区：[1]西安交通大学第二附属医院,710004

出　　处：《中华皮肤科杂志》2012年第5期364-365,共2页Chinese Journal of Dermatology

基　　金：西安交通大学国际交流项目（081430002）

摘　　要：目的探讨C3d在石蜡包埋的大疱性类天疱疮患者皮损组织中的表达及临床意义。方法免疫组化sP法在25例大疱性类天疱疮、10例大疱性表皮松解症及10例正常成人皮肤组织标本中进行C3d、IsG、IgA进行检测，并对其在大疱性类天疱疮皮损中阳性率进行比较。结果大疱性类天疱疮皮损中C3d、IgG、tgA的阳性率分别为96％、72％、0％。C3d、IgG在BP组织中表达阳性率的差异有统计学意义（Х^2=4．17，P〈0．05），C3d、IgA在BP组织中表达阳性率的差异有统计学意义（Х^2=22．04，P〈0．01）。C3d、IgG、IgA在10例EB及正常成人皮肤组织标本中表达均为阴性。结论免疫组化方法检测C3d的表达可以协助在石蜡组织中进行大疱性类天疱疮的诊断。Objective To study the expression of C3d in bullous pemphigoid lesions and its clinical significance. Methods The immunohistochemieal SP method was used to detect the expression of C3d, IgG and IgA in tissue specimens from the lesions of 25 patients with buUous pemphigoid, 10 patients with epidermolysis bullosa and normal skin of 10 human controls. The expression rates of these proteins were compared. Results The expression rates of C3d, IgG and IgA were 96%, 72.0% and 0 respectively in bullous pemphigoid tissue. Significant differences were found in the expression rates between C3d and IgG （X^2 = 4.17,P 〈 0.05）, as well as between C3d and IgA （X^2 = 22.04, P 〈 0.01 ）, in bullous pemphigoid tissue. No expression of C3d, IgG or IgA was observed in epidermolysis bullosa lesions or the control skin. Conslusion The detection of C3d with immunohistochemistry in paraffin-embedded tissue sections may facilitate the diagnosis of bullous pemphigoid.

关 键 词：大疱性类天疱疮 皮损组织 C3D 患者 大疱性表皮松解症 皮肤组织标本 免疫组化SP法 免疫组化方法 

分 类 号：R758.66[医药卫生—皮肤病学与性病学]