p53^(PIN3)基因多态性与膀胱移行细胞癌发病风险关系的研究  

Association of p53^(PIN3) polymorphism and susceptibities in bladder cancer

作　　者：田沛[1] 魏金星[1] 张雪培[1] 王智勇[1] 

机构地区：[1]郑州大学第一附属医院泌尿外科,郑州450052

出　　处：《临床泌尿外科杂志》2012年第5期339-341,共3页Journal of Clinical Urology

摘　　要：目的:探讨p53第3内含子16bp插入/缺失多态性与膀胱移行细胞癌发病风险的关系。方法:采用序列特异性引物,以PCR方法检测90例膀胱移行细胞癌患者和110例健康对照个体外周血DNA p53基因第3内含子的基因型。结果:膀胱癌组与对照组p53基因第3内含子16bp插入或缺失序列(PIN3)的A、A′等位基因频率分别83.33%、11.67%及96.36%、3.64%,两组比较差异有统计学意义(P<0.05);两组中三种基因型频率分别为76.67%、23.33%、0和92.73%、7.27%、0,分布差异有统计学意义(P<0.05)。膀胱移行细胞癌按临床分期比较:浅表性癌、浸润性癌突变与野生基因型差异有统计学意义(P<0.05);按病理分级比较:G1～G2、G3突变与野生基因型差异有统计学意义(P<0.05);按性别和年龄分组:两组突变与野生基因型频率比较差异均无统计学意义(P>0.05)。结论:p53第3内含子16bp插入/缺失多态性与膀胱癌发病风险存在相关性,可能是膀胱移行细胞癌患病的易感基因。Objective： To investigate the association of p53PIN3 gene polymorphism with the susceptibility to bladder cancer. Methods：The p53PIN3 genotypes were determined by PCR with sequence specific primers in 90 blad- der cancer patients and 110 healthy controls. Results：The frequency of A,Arallelotype among bladder cancer pa- tients and healthy controls were 83.33%, 11.67% and 96.36 % ,3.64 %, there were significant difference between two groups（P〈0. 05） ; The genotype frequency of AA,AA＇,A＇Ar were 76.67%,23.33%,0 和 92. 73%,7. 27%, 0, there was was significantly different between bladder cancer and healthy control （P〈 0.05） . When were grouped according to tumor stage, there were significant difference of mutant and wild genotype in Superficial carcinoma and invasive carcinoma. When the bladder cancer patients were grouped according to tumor grade, G1-G2 and G3 mutant compared to wild genotype, there was significant difference （P^0.05）. When the bladder cancer patients were grouped according to age and sex, there was no significant difference between two groups （P~ 0.05）. Conclusions：p53PIN3 gene polymorphism associated with development of bladder cancer, and may be a risk factor for the susceptibility of the bladder cancer.

关 键 词：膀胱移行细胞癌 P53基因 多态现象 

分 类 号：R737.14[医药卫生—肿瘤]