HGF对人胶质瘤新生血管形成的影响研究  

Influence of hepatocyte growth factor in neovascularization of human glioma

作　　者：郭友逢[1] 张萌[1] 李冰[1] 江晓珍[1] 李智[1] 

机构地区：[1]中山大学附属第一医院病理科,广州510080

出　　处：《中华神经医学杂志》2012年第5期469-472,共4页Chinese Journal of Neuromedicine

基　　金：广东省自然科学基金（201080609001041

摘　　要：目的探讨肝细胞生长因子（HGF）在人胶质瘤新生血管形成中的作用及可能机制。方法中山大学附属第一医院病理科自2002年1月至2008年6月共确诊胶质瘤患者72例，其中I级19例，Ⅱ级18例，Ⅲ级20例，Ⅳ级15例，免疫组化染色检测胶质瘤组织HGF、CD34的表达。体外常规培养胶质瘤细胞株U87MG，将荧光标记的HGFsi—RNA（si—HGF）特异性干扰序列转染至细胞内为转染si—HGF组，同时设正常对照组和阴性转染组作为对照：Westernblotting检测转染后细胞HGF、血管内皮生长因子（VEGF）的表达；将3组细胞悬液接种于鸡胚尿囊膜（CAM），5d后观察各组移植瘤周围血管形成情况。结果低级别胶质瘤（I级、Ⅱ级）HGF阳性率低于高级别胶质瘤（Ⅲ级、Ⅳ级），HGF阳性胶质瘤中微血管密度（MVD）高于HGF阴性胶质瘤，差异均有统计学意义（P〈0．05）。Westernboltting检测结果显示，与正常对照组和阴性转染组比较，转染si—HGF组细胞HGF的表达下降约76％．VEGF的表达下降约53％；移植瘤实验显示转染si—HGF组新生血管数、血管面积及血管生成面积比均低于正常对照组和阴性转染组组，差异有统计学意义（P〈0．05）。结论HGF与胶质瘤新生血管的形成关系密切，提示其有可能成为今后抗肿瘤血管生成的一个靶点。Objective To investigate the effect of hepatocyte growth factor （HGF） on the neovascularization of human glioma and its mechanism. Methods Seventy-two patients with gliomas, admitted to our hospital from January 2002 to June 2008, including 19 with grade I, 18 with grade II, 20 with grade Ⅲ and 15 with grade IV, were chosen in our study. HGF and CD34 expressions in the paraffin section ofgliomas in these 72 patients were detected with immunohistochemistry. Fluorescently labeled HGF-siRNA was transfected into U87MG glioma cells with TurboFect siRNA Transfection Reagent as si-HGF transfection group, and normal control group and negative transfection group were employed as controls; the HGF and vascular endothelial growth factor （VEGF） expressions in these U87MG cells were detected by Western blotting; cells from the 3 groups were inoculated to chorioallantoic membrane （CAM）, and the angiogenesis ability of these U87MG cells were determined 5 d later. Results HGF-positive rate in patients with low-grade gliomas （grade I and II） was significantly lower than that in patients with high-grade glioma （grade Ⅲ and IV, P〈0.05）; micro-vascular density （MVD） in gliomas with positive HGF was obviously higher than that in gliomas with negative HGF （P〈0.05）. As compared with those in cells of the normal control group and negative transfection group, the HGF and VEGF expressions in cells of the si-HGF transfeetion group were descended by 76% and 53%, respectively. The number and area of new vessels and the neovascularization ratio in cells of the si-HGF transfection group were signficantly decreased as compared with those in cells of the normal control group and negative transfection group （P〈0.05）. Conclusion Close relation exists between HGF and neovascularization of gliomas, which indicates that HGF might become a new target for future antiangiogenesis treatment.

关 键 词：神经胶质瘤: 肝细胞生长因子 血管形成 

分 类 号：R739.41[医药卫生—肿瘤]