杏临床仁研究中央核内注射NMDA型谷氨酸受体激动剂NMDA及拮抗剂D-AP-5引起摄水量而不是摄食量的改变(英文)  

Microinjection of NMDA-type glutamate receptor agonist NMDA and antagonist D-AP-5 into the central nucleus of the amygdale alters water intake rather than food intake

作　　者：闫君宝[1] 闫剑群[1,2] 李金容[1] 陈坷[1] 孙慧玲[1] 张嫄[1] 赵小林[1] 孙波[1] 赵师儒[1] 宋琳[1] 魏晓静[1] 

机构地区：[1]西安交通大学医学院生理及病理生理系,陕西西安710061 [2]西安交通大学医学院教育部环境与疾病相关基因重点实验室,陕西西安710061

出　　处：《南方医科大学学报》2012年第5期595-600,共6页Journal of Southern Medical University

基　　金：Supported by National Natural Science Foundation of China(30970973;31000518;31171052)~~

摘　　要：目的探讨杏仁中央核中的N-甲基-D-天冬氨酸（NMDA）型谷氨酸受体对食物和水摄取的影响。方法不锈钢套管植入雄性Sprague-Dawley大鼠一侧杏仁中央核内,NMDA型谷氨酸受体激动剂原型物NMDA,或选择性NMDA受体拮抗剂D（）-2-氨基-5-磷酰基戊酸（D-AP-5）微量注射入饱食和含水度正常的大鼠的杏仁中央核内。结果杏仁中央核内注射入8.50,17.00,或34.00 nmol NMDA不改变大鼠摄食量,但是增加注射后0~1 h内不依赖于食物摄取的摄水量（F3,32=3.191,P=0.037）。杏仁中央核内注射入6.34,12.70,或25.40 nmol D-AP-5也不影响大鼠摄食量,但是减少了注射后0~1 h内不依赖于食物摄取的摄水量（F3,28=3.118,P=0.042）。结论杏仁中央核中的NMDA受体可能参与了对摄水而不是摄食的调控。Objective To investigate the role of N-Methyl-D-aspartic acid （NMDA）-type glutamate receptors in the central nucleus of the amygdale （CeA） in food and water intake. Methods Male Sprague-Dawley rats with stainless steel cannulae implanted unilaterally into the CeA were used. The prototypic NMDA receptor agonist NMDA, or the selective NMDA receptor antagonist D（-）-2-amino-5-phosphonopentanoic acid （D-AP-5） was microinjected into the CeA of satiated and euhydrated rats. Results Intra-CeA injection of 8.50, 17.00, or 34.00 nmol NMDA did not alter food intake but significantly increased water intake 0-1 h after the injection （F~=3,191, P=0.037） independent of food intake. Without affecting the food intake, injection of 6.34, 12.70, or 25.40 nmol D-AP-5 into the CeA significantly decreased water intake 0-1 h after the injection （F3.~=3.118, P=0.042） independent of food intake. Conclusion NMDA receptors in the CeA may participate in the control of water intake rather than food intake.

关 键 词：NMDA受体 NMDA D-AP-5 杏仁中央核 食物摄取 水摄取 

分 类 号：R338[医药卫生—人体生理学]