八探针间期荧光原位杂交技术联合染色体核型分析比较成人与儿童急性淋巴细胞白血病患者的细胞遗传学差异  

Cytogenetic differences between adults and children with acute lymphoblastic leukemia: eight-probe fluorescence in situ hybridization and karyotype analyses

作　　者：左媛[1,2] 杜庆锋[1] 李荣[1] 许娜[1] 曹睿[1] 廖立斌[1] 许露露[1] 张晋芳[1] 黄彬涛[1] 骆许静[1] 肖晓珍[1] 刘晓力[1] 

机构地区：[1]南方医科大学南方医院血液科,广东广州510515 [2]内蒙古医学院第二附属医院检验科,内蒙古呼和浩特010030

出　　处：《南方医科大学学报》2012年第5期707-709,共3页Journal of Southern Medical University

基　　金：国家自然科学基金(81170521)~~

摘　　要：目的应用八探针间期荧光原位杂交技术(FISH)联合染色体核型分析观察急性淋巴细胞白血病(ALL)成人患者与儿童患者的细胞遗传学差异。方法对125例ALL患者(成人86例、儿童39例)全部行八探针FISH(MYC、P16、E2A、TEL/AML1、BCR/ABL、MLL、IGH、多倍体的DNA探针)检测并染色体核型分析。结果八探针FISH检测结果显示,成人ALL患者与儿童ALL患者的TEL/AML1融合基因、BCR/ABL融合基因与多倍体阳性率之间的差异有统计学意义(P<0.05);染色体核型分析成人ALL患者与儿童ALL患者的(t9;22)易位、多倍体阳性率之间的差异有统计学意义(P<0.05)。结论成人ALL患者与儿童ALL患者融合基因表达各有侧重,不同的细胞遗传学特征与其预后密切相关。FISH多探针诊断系统检测ALL患者常见遗传学异常省时、准确、高效,与染色体核型分析形成很好的互补。Objective To investigate the cytogenetic differences between children and adults with acute lymphoblastic leukemia（ALL） using eight-probe fluorescence in situ hybridization and karyotype analysis.Methods Eight-probe（MYC,P16,E2A,TEL/AML1,BCR/ABL,MLL,IGH,and hyperdiploidy） fluorescence in situ hybridization and karyotype analysis were performed for 86 adults and 39 children with acute lymphoblastic leukemia.Results Eight-probe fluorescence in situ hybridization showed significant differences in the positivity rate of TEL/AML1,BCR/ABL,and hyperdiploidy between adult patients and children with ALL.By karyotype analysis,the positivity rate of t（9;22） and hyperdiploidy differed significantly between the children and adult patients（P0.05）.Conclusion Adults and children with ALL have different expression profiles of the fusion genes.Eight-probe fluorescence in situ hybridization is time-saving,accurate and efficient in detecting common genetic abnormalities in ALL patients,and can be well complementary to karyotype analysis in clinical diagnosis of ALL.

关 键 词：八探针FISH 染色体核型分析 急性淋巴细胞白血病 细胞遗传学 

分 类 号：R733.71[医药卫生—肿瘤]