富含12-MTA的嗜碱芽孢杆菌X1的鉴定及其脂肪酸组成分析  

Identification and fatty acid composition analysis of 12-MTA-rich alkaliphilic Bacillussp X1

作　　者：李宏彬[1] 陈三凤[2] 

机构地区：[1]南阳理工学院生物与化学工程系,河南南阳473004 [2]中国农业大学农业生物技术国家重点实验室,北京100094

出　　处：《中国油脂》2012年第5期83-87,共5页China Oils and Fats

基　　金：国家自然科学基金项目资助(30670036);南阳理工学院博士科研启动金资助项目(BS200801)

摘　　要：12-甲基十四烷酸(12-MTA,anteiso-C15∶0)具有显著的抗肿瘤活性。以重度盐碱土中分离的产芽孢细菌X1为研究对象,通过16S rDNA系统发育分析和表型特征确定其分类地位,通过细胞裂解、脂肪皂化、盐酸催化的甲酯化和萃取等步骤制备X1菌体总脂肪酸甲酯衍生物,GC-MS分析其组成,结合称重法测定X1菌体总脂肪酸甲酯衍生物净重。结果表明:菌株X1为芽孢杆菌属(Bacillus)的成员,其16S rDNA相似性数据显示其为芽孢杆菌属内的一个新种。GC-MS分析确定X1菌体主要含有iso-C14∶0、C14∶0i、so-C15∶0和anteiso-C15∶0 4种脂肪酸,其中anteiso-C15∶0(12-MTA)比例占到X1菌体总脂肪酸含量的76.2%,含量占X1菌体干重的4.47%。12 - methyltetradecanoic acid （ 12 - MTA, anteiso - C15：0 ） possesses notable antitumor activity. The spore - producing bacterium X1 isolated from severe saline - alkali soil was taken as research ob- ject, and its taxonomic position was determined by 16S rDNA -based phylogenetic analysis and phenotypic characteristics. The total fatty acid methyl ester derivatives of X1 were prepared through cell lysis, fat saponification, HC1 -catalyzed methyl esterification and extraction, and their compositions were analyzed by GC - MS. The net weight of total fatty acid methyl ester derivatives of X1 was determined by weighing method. The results showed that the strain X1 was a member of genus Bacillus, and the 16S rDNA similarity data showed it should be a novel species within the genus Bacillus. GC - MS analysis determined that fatty acids of X1 mainly contained four kinds of fatty acids which were iso -C1,0 ,C14：o ,iso- C15：0 and anteiso - C15：0, of which anteiso - C15：0（ 12 - MTA） was 76.2% of the total fatty acid content of X1 and 4.47% of X1 dry weight.

关 键 词：芽孢杆菌 脂肪酸 12-甲基十四烷酸 抗肿瘤 

分 类 号：Q93[生物学—微生物学]