适量运动和甲状腺激素对大鼠肝脏氧化应激反应的影响  

Effects of Moderate Exercise and Thyroid Hormone Adding on Oxidative Stress of Liver in Rats

作　　者：崔晗[1] 卢沛琦[2] 叶振坤[3] 周伟民[3] 陈赛阁[1] 徐书祥[1] 张帅[1] 潘亮[3] 杜艳姣[3] 朱峰[3] 贾敏[3] 

机构地区：[1]郑州大学检验系,河南郑州450052 [2]郑州大学第二附属医院 [3]郑州大学基础医学院

出　　处：《环境与健康杂志》2012年第5期407-409,共3页Journal of Environment and Health

基　　金：全国大学生创新创业训练计划(121045963);河南省博士后科研启动基金;国家自然科学基金重点项目(30230330);国家自然科学基金面上项目(30850003);卫生部基金(2005-01);河南省科技攻关计划项目(112102310680)

摘　　要：目的观察适量运动和甲状腺激素对大鼠肝脏H_2O_2含量和抗氧化能力的影响。方法将64只Wistar大鼠随机分为8组:对照组(C)、轻度甲亢组(T_1)、重度甲亢组(T_2)、甲减组(J)、运动对照组(E)、轻度甲亢运动组(ET-1)、重度甲亢运动组(ET-2)、甲减运动组(EJ)。C组生理盐水灌胃,T_1和T_2组分别给予左旋甲状腺素钠100和200μg/d灌胃,J组给予甲巯咪唑10 mg/d灌胃,运动为负重6%体重游泳训练30 min/d。喂养14 d后,检测肝组织匀浆中的H_2O_2含量、谷胱甘肽过氧化酶(GPx)活力、超氧化物歧化酶(SOD)活力以及丙二醛(MDA)含量。结果非运动条件下,甲亢组与对照组比较,H_2O_2、GPx、SOD和MDA水平均增高,差异有统计学意义(P<0.05);甲减组与对照组比较,H_2O_2、GPx、SOD和MDA水平均降低,差异有统计学意义(P<0.05);甲亢运动组和甲减运动组与运动对照组比较,H_2O_2、GPx、SOD和MDA水平均增高,差异有统计学意义(P<0.05);甲状腺激素水平异常的运动组与对应非运动组比较,H_2O_2、GPx、SOD和MDA水平均增高,差异有统计学意义(P<0.05);而甲状腺激素水平正常的运动对照组与对应非运动组比较,MDA差异无统计学意义(P>0.05)。结论运动和甲亢均可诱发大鼠肝脏H_2O_2含量和抗氧化酶活力增高,二者有一定的协同作用;甲亢、甲亢合并运动和甲减合并运动均会诱发失代偿性氧化损伤,单纯的适量运动不会诱发氧化损伤。Objective To study the effects of moderate exercise and thyroid hormone adding on the liver H2O2 content and anti-oxidative capability in rats. Methods Wistar rats were randomly divided into eight groups, control（C）, mild hyperthyroidism （TI）, severe hyperthyroidism （T2） and hypothyroidism （J）, furthermore, each group were divided into two groups, such as exercise and no exercise. The model of mild hyperthyroidism（T1）, severe hyperthyroidism（T2） and hypothyroidism（J） rats were established by giving 100 μg/d levothyroxine sodium （L-T4）, 200μg/d L-T4 and 10 mg/d methimazole respectively. After 14 d of treatment, the rats were sacrificed and liver H202 content, GPx activity, SOD activity and MDA content were measured. Results All of the H2O2 content, GPx activity, SOD activity and MDA content were higher in hyperthyroidism group, and which were lower in hypothyroidism group. Furthermore, given by levothyroxine or methimazole, all indexes were higher in exercise group than those of no exercise group, however, there was no difference in the MDA content between exercise group and control group. Conclusion Both exercise and hyperthyroidism may induce the increasing of H2O2 content and the anti-oxidative capability in the liver of rats, and the synergistic effect has been observed. Exercise plus hyperthyroidism, exercise plus hypothyroidism and hyperthyroidism may induce oxidative damage.

关 键 词：运动 甲状腺激素 过氧化氢 抗氧化能力 肝脏 大鼠 

分 类 号：R994.6[医药卫生—毒理学]