A/O MBR处理生活污水效率与菌群多样性的关系  

Relationship Between Sewage Treatment Efficiency and Bacterial Community Diversity in an A/O MBR

作　　者：邝斌宇[1] 史青[2] Montcho Leon Monthero 丁嫚[1] 温东辉[1] 

机构地区：[1]北京大学环境科学与工程学院,北京100871 [2]中国地质大学(北京)水资源与环境学院,北京100083

出　　处：《环境科学》2012年第6期2061-2067,共7页Environmental Science

基　　金：国家水体污染控制与治理科技重大专项(2009ZX07102-002-01)

摘　　要：采用缺氧/好氧膜生物反应器(A/O MBR)工艺处理生活污水,试运行了5种不同的工况以确定最佳工艺参数,利用变性凝胶梯度电泳(DGGE)技术研究缺氧池和好氧池(MBR)中细菌群落结构,分析出水水质与细菌种群多样性的关系.结果表明A/O MBR在水力停留时间(HRT)为12 h,污泥停留时间(SRT)为10 d,硝化液回流比为300%,污泥回流比为100%的条件下,对COD、氨氮、总氮有稳定良好的去除,平均去除率分别为96.4%、99.1%、75.8%;系统运行过程中,缺氧池和好氧池中菌群结构发生较大变化,同一工况下两池菌群的相似性通常大于50%,但缺氧池菌群多样性随工况变换而波动较大,好氧池菌群多样性随运行时间而逐渐丰富;缺氧池菌群多样性指数与反硝化效率成正相关关系.An Anoxic/Oxic Membrane Bioreactor(A/O MBR) was used to treat sewage.Five different working conditions were run to determine the optimal process parameters.Bacterial community structures in both anoxic and oxic tanks were analyzed using denaturing gradient gel electrophoresis(DGGE).The relationship between effluent water quality and bacterial community diversity was established.The experimental results indicated that,under the optimal parameters of hydraulic retention time(HRT) 12 h,sludge retention time(SRT) 10 d,reflux ratio of nitrified effluent 300%,and reflux ratio of sludge 100%,the A/O MBR removed COD,NH+4-N,and TN effectively and stably with the average removal rates of 96.4%,99.1% and 75.8%,respectively.The bacterial communities varied markedly in both anoxic and oxic tanks during the sewage treatment experiment.Under a same working condition,the communities in both tanks were often similar with a similarity of more than 50%.The community diversity of the anoxic tank fluctuated depending on different working conditions,while the diversity of the oxic tank increased steadily along with the operation time.A positive correlation between the bacterial community diversity of the anoxic tank and the denitrification efficiency of the A/O MBR was established.

关 键 词：A/OMBR 菌群多样性 工艺优化 脱氮 DGGE 

分 类 号：X172[环境科学与工程—环境科学] X703.1