体外二氧化碳气腹对膀胱癌黏附分子表达的影响  

Effect of CO_2 pneumoperitoneum on bladder carcinoma cell adhesion molecules expression in vitro

作　　者：孙方浒[1] 洪涛[1] 王仙友 朱汝健 蔡仙国[1] 张文刚[1] 俞洪元[1] 柯莽[1] 

机构地区：[1]浙江省台州医院泌尿外科,浙江台州317000 [2]浙江台州恩泽医疗中心(集团)路桥医院泌尿外科,浙江台州317000

出　　处：《中国内镜杂志》2012年第4期346-349,共4页China Journal of Endoscopy

摘　　要：目的探讨二氧化碳(CO2)气腹对膀胱癌细胞黏附分子表达的影响。方法体外培养膀胱癌T24细胞,以15mmHgCO2压力,作用于癌细胞2、4和6h,以免疫细胞化学法检测各组T24细胞株E-钙调素,整合素α6β4、α2β1,ICAM-1及CD44V6等蛋白的表达。结果体外模拟CO2气腹对T24细胞体外黏附细胞分子表达有影响:ICAM-1、CD44V6表达升高(均为P<0.01);并与作用时间呈相关(均为P<0.05);E-钙调素的表达减少(P<0.01),随CO2气腹作用时间呈负相关(P<0.05)。整合素α6β4、α2β1的表达明显改变(均为P>0.05)。结论 CO2气腹环境可引起体外培养的膀胱癌细胞黏附细胞分子表达改变,这可能促进癌细胞扩散转移的能力。[Objective] To investigate the effects of carbon dioxide（CO2） pneumoperitoneum on cell adhesion molecules expression of human bladder carcinoma cells.[Methods] The cultured T24 cell line were incubated in vitro with CO2 insufflations at pressures 15 mmHg,and the time of treatment for each pressure were 2 h,4 h and 6 h respectively.The changes of adhesion molecules（E-cadherin,integrinα6β4 and α2β1,ICAM-1,CD44） in T24 cell line were detected by immunohistochemistry method respectively.[Results] The CO2 pneumoperitoneum were the factor influencing some adhesion molecules of the T24 cells.The expression of ICAM-1,CD44 were found increased（P 0.01,respectively）,and there was a positive correlation with the CO2 treatment time（P 0.05,respectively）.The expression of E-cad was decreased,and it was negative correlated with treatment time.The expression of integrin α6β4 and α2β1 were found no statistically significant change respectively（P 0.05）.[Conclusion] Carbon dioxide pneumoperitoneum can make change on some adhesion molecules＇ expression,and that may increase the capability of invasion and metastasis in the laparoscopic therapy on bladder carcinoma.

关 键 词：气腹 二氧化碳 膀胱癌 黏附 侵袭 

分 类 号：R743.31[医药卫生—神经病学与精神病学] R737.11[医药卫生—临床医学]