荧光灶试验和TCID_(50)-ELISA方法在Ⅲ价轮状病毒重配疫苗感染滴度测定中的对比研究  

Comparison of fluorescent focus assay and TCID_(50)-ELISA method for detecting infection titer of trivalent rotavirus reassortant vaccine

作　　者：王名强[1] 余黎[1] 安红[1] 崔焰[1] 韩平[1] 包红[1] 寇桂英[1] 周旭[2] 

机构地区：[1]兰州生物制品研究所有限责任公司,兰州730046 [2]上海生物制品研究所有限责任公司,上海200052

出　　处：《中国新药杂志》2012年第10期1166-1169,共4页Chinese Journal of New Drugs

基　　金：国家科技支撑计划(2008BAI66B08)

摘　　要：目的:对比荧光灶试验和TCID50-ELISA方法测定III价轮状病毒基因重配疫苗感染滴度的精确性,重复性和可行性。方法:同时采用TCID50-ELISA方法和荧光灶试验方法测定Ⅲ价轮状病毒基因重配疫苗的感染滴度,对测定结果进行比较分析。结果与结论:在Ⅲ价轮状病毒基因重配疫苗的感染滴度测定中,荧光灶试验方法和TCID50-ELISA方法各有其特点,荧光灶试验方法更加精确和快速,相对于TCID50-ELISA方法,具有更低的变异性,即其重复性较高;而TCID50-ELISA方法受主观因素的影响较低。Objective： To compare the accuracy, reproducibility, and applicability of fluorescent focus assay and TCID50-ELISA method for detecting infection titer of trivalent rotavirus reassortant vaccine. Methods： TCID50-ELISA method and fluorescent focus assay method were used to detect the infection titer of trivalent rotavirus reassortant vaccine. Test results were compared. Results and Conclusion： Each method for infectious titer of rota- virus has its own characteristics. Fluorescent focus assay is more accurate and faster than TCID50-ELISA method. Compared with TCID50-ELISA method, fluorescent focus assay has distinctly lower variance than TCID50-ELISA method, in other words, it has the finer reproducibility. Whereas the decision of mantissa by the fluorescent focus assay is more subjective than by TCID50-ELISA.

关 键 词：TCID50-ELISA 荧光灶试验 III价轮状病毒基因重配疫苗 感染滴度 

分 类 号：R186[医药卫生—流行病学]