^(60)Co-γ射线诱变小麦品质突变体的筛选及分子标记检测  

Screening Quality Mutants of Wheat Radiated by ^(60)Co-γ Ray and Molecular Identification

作　　者：来德娥[1] 赵平[1] 王敏[1] 张从宇[2] 

机构地区：[1]安徽农业大学农学院安徽作物生物学省级实验室,合肥230036 [2]安徽科技学院,安徽凤阳233100

出　　处：《种子》2012年第5期5-10,共6页Seed

基　　金：国家科技支撑计划课题(编号:2006 BAD 01 A 02);安徽作物生物学省级实验室项目(编号:10111406024)

摘　　要：为了探索小麦品质性状的物理诱变效应,用300 Gy的60Co-γ射线辐射小麦品种河科2号的干种子,对M3代234个株系的蛋白质含量、湿面筋含量、沉降值、硬度和产量性状进行变异分析和SSR分子鉴定,以超过均值±2×标准差确定变异株系。结果表明,在M3代群体中,蛋白质含量有13个株系发生正向变异,5个株系发生负向变异;湿面筋含量有8个株系发生正向变异,3个株系发生负向变异;沉降值有6个株系发生正向变异,3个株系发生负向变异;硬度有2个株系发生正向变异,9个株系发生负向变异;产量有5个株系发生正向变异,4个株系发生负向变异。相关分析表明,M3群体蛋白质含量、湿面筋含量、沉降值呈极显著正相关,表现出平行变异的趋势。初步筛选出蛋白质含量和湿面筋含量符合国家强筋小麦标准(蛋白质含量≥15%,湿面筋含量≥35%)的株系8个。经SSR分子标记检测,与控制小麦蛋白质含量的基因存在连锁的2个特异引物Xbarc 164和Xgwm 161在以上8个变异株系都呈现多态性标记,表明这些株系与蛋白质含量相关的基因可能发生了变异。To clarify the mutagenic effects of radiation to quality characters of wheat,dry seeds of wheat variety Heke 2 were radiated by 60Co-γ ray with the dose of 300 Gy. The genetic variation of 234 M3 lines on protein content, wet gluten content, sedimentation value, hardness and yield were analyzed, and the mutants were identified by molecular marker. Depending on the criterion of over mean ±2 s, 13 positive mutant lines and 5 negative mutant lines in protein content,8 positive mutant lines and 3 negative mutant lines in wet gluten content, 6 positive mutant lines and 3 negative mutant lines in sedimentation value,2 positive mutant lines and 9 negative mutant lines in hardness, and 5 positive mutant lines and 4 negative mutant lines in yield were identified from 234 M3 lines, respectively. Significant correlation of protein, gluten and sedimentation content indicated parallel variations might be occurred in these traits. Based on national criterion （protein content ≥ 15%, wet gluten content≥35% ）,8 lines with expected protein and wet gluten content were preliminarily selected. The two SSR primers of Xbarc 164 and Xgwm 161 correlated with protein content presented polymorphism in those 8 mutant lines by molecular marker, the genetic loci of protein content could be occurred.

关 键 词：小麦 60Co-γ射线 蛋白质含量 湿面筋含量 沉降值 硬度 产量 SSR分子标记 

分 类 号：S512.1[农业科学—作物学]